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Related Experiment Video

Updated: Apr 28, 2026

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
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A sensitive gold nanoparticle-based colorimetric aptasensor for Staphylococcus aureus.

Jinglei Yuan1, Shijia Wu1, Nuo Duan1

  • 1State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

Talanta
|June 11, 2014
PubMed
Summary

A new colorimetric aptasensor detects Staphylococcus aureus (S. aureus) using gold nanoparticles and tyramine signal amplification. This method achieves a low limit of detection for S. aureus, offering a sensitive diagnostic tool.

Keywords:
AptamerGold nanoparticlesStaphylococcus aureusTyramine signal amplification

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Area of Science:

  • Nanotechnology
  • Biosensors
  • Analytical Chemistry

Background:

  • Staphylococcus aureus (S. aureus) is a significant pathogen.
  • Rapid and sensitive detection methods are crucial for S. aureus identification.
  • Existing detection methods may lack sensitivity or speed.

Purpose of the Study:

  • To develop a gold nanoparticle-based colorimetric aptasensor for S. aureus detection.
  • To utilize tyramine signal amplification (TSA) technology for enhanced sensitivity.
  • To establish a quantitative detection method for S. aureus.

Main Methods:

  • Immobilization of biotinylated aptamer specific for S. aureus on microtiter plates.
  • Sequential addition of S. aureus, aptamer-streptavidin-HRP conjugates, biotinylated tyramine, hydrogen peroxide, and avidin-catalase.
  • Colorimetric detection based on gold (III) chloride trihydrate reaction and absorbance measurement at 550 nm.

Main Results:

  • A linear relationship between absorbance at 550 nm and S. aureus concentration (10 to 10^6 cfu mL⁻¹) was observed (R² = 0.9947).
  • The developed aptasensor demonstrated a limit of detection of 9 cfu mL⁻¹.
  • The method provides a sensitive and quantitative approach for S. aureus detection.

Conclusions:

  • The developed gold nanoparticle-based colorimetric aptasensor with TSA is effective for S. aureus detection.
  • The method offers high sensitivity and a low limit of detection.
  • This aptasensor shows potential for rapid and accurate S. aureus diagnostics.