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Coherent convergent-beam time-resolved X-ray diffraction.

John C H Spence1, Nadia A Zatsepin2, Chufeng Li2

  • 1Department of Physics, Arizona State University, Tempe, AZ 85287-1504, USA spence@asu.edu.

Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
|June 11, 2014
PubMed
Summary
This summary is machine-generated.

Coherent X-ray lasers enable new protein structure analysis using nanometre beams. This study explores coherent convergent-beam (CCB) patterns for time-resolved crystallography and phase determination in structural biology.

Keywords:
coherent convergent beamcoherent nanodiffractionfree-electron X-ray laserphase determinationtime-resolved diffraction

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Area of Science:

  • Structural Biology
  • Crystallography
  • X-ray Optics

Background:

  • Coherent X-ray lasers offer nanometre beam diameters and large divergence for structural biology.
  • Application to protein nanocrystals and single particles presents unique challenges and opportunities.

Purpose of the Study:

  • To discuss coherent convergent-beam (CCB) hard X-ray diffraction patterns.
  • To explore CCB patterns for time-resolved crystallography with monochromatic free-electron X-ray lasers.
  • To investigate obtaining single-shot, angle-integrated rocking curves from CCB patterns.

Main Methods:

  • Analysis of CCB hard X-ray diffraction patterns.
  • Investigating the dependence of diffraction patterns on focused beam coordinates.
  • Examining structure factor phase information from overlapping interfering orders.

Main Results:

  • CCB patterns offer potential for time-resolved crystallography, overcoming limitations of monochromatic laser radiation.
  • Single-shot, angle-integrated rocking curves can be obtained from CCB patterns.
  • Structure factor phase information is accessible through overlapping orders, enabling common phase origin determination.

Conclusions:

  • CCB hard X-ray diffraction patterns present a novel approach for structural biology.
  • The methods discussed facilitate phase retrieval and refinement in nanocrystal and single-particle analysis.
  • This technique holds promise for advancing time-resolved crystallography and structural determination.