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Alloantibody to a Bw4 epitope in a Bw4+B*27: 05 patient
Charles T Lutz1, Ahmad Al-Attar, John R May
11 Department of Pathology and Laboratory Medicine, University of Kentucky, Lexington, KY. 2 Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY. 3 Address correspondence to: Charles T. Lutz, M.D., Ph.D., Department of Pathology and Laboratory Medicine, University of Kentucky, 800 Rose Street, MS 117, Lexington, KY 40536-0298.
Background:
Alloantibodies to the Bw4 epitope are known to be heterogeneous, but it is widely assumed that anti-Bw4 alloantibodies arise only in individuals who do not express a Bw4 epitope.
Methods:
Bw4 expression was confirmed by DNA sequence analysis. Anti-Bw4 reactivity was confirmed by absorption with transfected cells.
Results:
A Bw4 (B*27:05 or B*27:13) patient expressed antibody that bound all Bw4 human leukocyte antigen-A and human leukocyte antigen-B antigens tested, except B*27:05 and B*44:02. Serum absorbed with B*51:01-transfected HYM2.C1R cells left only reactivity to B17 (B57, B58), but not to any other Bw4 antigens.
Conclusion:
A Bw4 patient made antibody to a Bw4 epitope. This finding indicates that apparent anti-Bw4 or anti-Bw6 antibody should not be ignored even in patients who express a common Bw4 or Bw6 antigen, respectively.
