Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Racial and ethnic disparities in clinical outcomes of HER2-positive metastatic breast cancer treated with antibody-drug conjugates.

Journal of the National Cancer Institute·2026
Same author

Retraction Note: Heterozygous deletion of chromosome 17p renders prostate cancer vulnerable to inhibition of RNA polymerase II.

Nature communications·2026
Same author

Test-Retest Reliability and Mode Effects on Single-Item and Multi-Item Measures in a Survey of Adolescent and Young Adults with Cancer.

Journal of adolescent and young adult oncology·2026
Same author

Identification of a Novel <i>GOLGB1-RET</i> Fusion in Papillary Thyroid Cancer With a Durable Response to Selpercatinib.

JCO precision oncology·2026
Same author

Integrated Molecular and Clinical Analysis of Thymic Epithelial Tumors.

JCO precision oncology·2026
Same author

Broader gene representation by whole-exome sequencing improves accuracy of tumor mutational burden assessment for selection of pembrolizumab immunotherapy.

Cancer immunology, immunotherapy : CII·2026

Related Experiment Video

Updated: Jan 9, 2026

Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells
09:32

Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells

Published on: February 27, 2020

9.5K

Phenotypic plasticity in normal breast derived epithelial cells.

Candice A M Sauder, Jillian E Koziel, MiRan Choi

  • 1Department of Surgery, Feinberg School of Medicine, Northwestern University, 303 E, Superior Street, Chicago, IL 60611, USA. susan.clare@northwestern.edu.

BMC Cell Biology
|June 12, 2014
PubMed
Summary

Normal breast epithelial cells isolated from the Susan G. Komen for the Cure® Tissue Bank show remarkable plasticity. These cells can differentiate into multiple cell types, offering insights into aggressive breast cancer origins.

More Related Videos

Three Dimensional Cultures: A Tool To Study Normal Acinar Architecture vs. Malignant Transformation Of Breast Cells
08:33

Three Dimensional Cultures: A Tool To Study Normal Acinar Architecture vs. Malignant Transformation Of Breast Cells

Published on: April 25, 2014

20.3K
Time-lapse Imaging of Primary Preneoplastic Mammary Epithelial Cells Derived from Genetically Engineered Mouse Models of Breast Cancer
11:47

Time-lapse Imaging of Primary Preneoplastic Mammary Epithelial Cells Derived from Genetically Engineered Mouse Models of Breast Cancer

Published on: February 8, 2013

12.4K

Related Experiment Videos

Last Updated: Jan 9, 2026

Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells
09:32

Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells

Published on: February 27, 2020

9.5K
Three Dimensional Cultures: A Tool To Study Normal Acinar Architecture vs. Malignant Transformation Of Breast Cells
08:33

Three Dimensional Cultures: A Tool To Study Normal Acinar Architecture vs. Malignant Transformation Of Breast Cells

Published on: April 25, 2014

20.3K
Time-lapse Imaging of Primary Preneoplastic Mammary Epithelial Cells Derived from Genetically Engineered Mouse Models of Breast Cancer
11:47

Time-lapse Imaging of Primary Preneoplastic Mammary Epithelial Cells Derived from Genetically Engineered Mouse Models of Breast Cancer

Published on: February 8, 2013

12.4K

Area of Science:

  • Cell Biology
  • Stem Cell Research
  • Cancer Biology

Background:

  • The Susan G. Komen for the Cure® Tissue Bank (KTB) provides normal human breast tissue for research.
  • Epithelial (K-HME) and stromal (K-HMS) cells were isolated using explant culture.
  • Focus on primary, non-transformed epithelial cells characterized via multiple methods.

Purpose of the Study:

  • To characterize normal human breast epithelial cells.
  • To investigate the differentiation potential of these cells.
  • To explore potential links to metaplastic carcinoma of the breast.

Main Methods:

  • Explant culture for cell isolation.
  • Selective media and trypsinization for cell selection.
  • Immunohistochemistry, flow cytometry, and in vitro cell culture for characterization.

Main Results:

  • Normal breast epithelial cells exhibit in vitro differentiation into diverse cell types (e.g., osteoclasts, chondrocytes, neural progenitors, muscle, melanocytes).
  • These cells express embryonic stem cell markers.
  • Demonstrated plasticity in vitro.

Conclusions:

  • The study identified pluri/multipotent epithelial cells through specific culture conditions.
  • The observed cellular plasticity may inform the origins of metaplastic carcinoma of the breast, an aggressive triple-negative breast cancer subtype.
  • Normal breast epithelial cells from the KTB are valuable research tools.