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Published on: December 12, 2017
Campylobacter jejuni gene cj0511 encodes a serine peptidase essential for colonisation
A V Karlyshev1, G Thacker2, M A Jones3
1The London School of Hygiene & Tropical Medicine, Keppel St, London WC1E 7HT, United Kingdom ; School of Life Sciences, Faculty of Science, Engineering and Computing, Kingston University, Kingston upon Thames, Penrhyn Road, KT1 2EE, United Kingdom.
Abstract:
According to MEROPS peptidase database, Campylobacter species encode 64 predicted peptidases. However, proteolytic properties of only a few of these proteins have been confirmed experimentally. In this study we identified and characterised a Campylobacter jejuni gene cj0511 encoding a novel peptidase. The proteolytic activity associated with this enzyme was demonstrated in cell lysates. Moreover, enzymatic studies conducted with a purified protein confirmed a prediction of it being a serine peptidase. Furthermore, cj0511 mutant was found to be severely attenuated in chicken colonisation model, suggesting a role of the Cj0511 protein in infection.
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