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Application of an electrochemical immunosensor with a MWCNT/PDAA modified electrode for detection of serum trypsin
Qiang Yi1, Qicai Liu2, Feng Gao3
1Department of Laboratory Medicine, the 1st Affiliated Hospital, Fujian Medical University, Fuzhou 350005, China. yiqiang8798@163.com.
Objective:
To establish an electrochemical immunosensor for the determination of serum trypsin levels using a multiwall carbon nanotubes (MWCNTs)-composite-modified electrode.
Method:
A MWCNT composite coated on the surface of bare gold electrodes was used for fixation of an anti-trypsin antibody. The assembly process and the performance indicators, including sensitivity, linear range of detection, anti-jamming performance, and stability, of the electrochemical immunosensor were examined by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS).
Results:
With optimized experimental conditions, the difference of the current value measured by differential pulse voltammetry (DPV) showed a linear relationship with the concentration of serum trypsin within 0.10-100 ng/mL. The detection limit for trypsin using this sensor was 0.002 ng/mL.
Conclusions:
The electrochemical immunosensor built using the MWCNT-composite-modified electrode is simple to operate and has a fast response time, along with a wide linear range, high sensitivity, and accuracy, making it suitable for serum trypsin detection.
