Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum to "Effective rapid screening for sulfoxaflor insecticide in ornamental flowers by paper-based biosensing formats implementing sustainable extraction and packaging approaches" [Talanta, 300 (2026) 129168].

Talanta·2026
Same author

Effective rapid screening for sulfoxaflor insecticide in ornamental flowers by paper-based biosensing formats implementing sustainable extraction and packaging approaches.

Talanta·2025
Same author

Rule-based algorithm for the identification of single and binary mixtures of cross-reacting neonicotinoids with microsphere-based immunoassays.

Talanta·2025
Same author

Discovery of multiple bee-hazardous pesticides in ornamental plants via the Bee-Plex multi-target microsphere screening method.

Journal of hazardous materials·2024
Same author

Smartcard: an integrated approach for contaminant monitoring, from field to laboratory.

Analytical and bioanalytical chemistry·2024
Same author

Bridging the Gap between Field Experiments and Machine Learning: The EC H2020 B-GOOD Project as a Case Study towards Automated Predictive Health Monitoring of Honey Bee Colonies.

Insects·2024

Related Experiment Video

Updated: Apr 28, 2026

ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection
12:11

ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection

Published on: October 23, 2009

13.5K

6-Plex microsphere immunoassay with imaging planar array detection for mycotoxins in barley.

Jeroen Peters1, Alice Cardall, Willem Haasnoot

  • 1RIKILT Wageningen UR (Institute of Food Safety), Akkermaalsbos 2, 6700 AE Wageningen, The Netherlands. jeroen.peters@wur.nl.

The Analyst
|June 13, 2014
PubMed
Summary

A new multiplex immunoassay rapidly screens for multiple mycotoxins in barley, offering a fast and reliable method for food safety. This assay detects aflatoxin B1, ochratoxin A, zearalenone, deoxynivalenol, T2-toxin, HT-2 toxin, and fumonisin B1.

More Related Videos

A Multiplex Serological Assay for the Detection of Antibody Responses to Arboviruses
05:22

A Multiplex Serological Assay for the Detection of Antibody Responses to Arboviruses

Published on: November 4, 2025

1.0K
Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles
08:50

Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

Published on: October 10, 2013

11.0K

Related Experiment Videos

Last Updated: Apr 28, 2026

ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection
12:11

ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection

Published on: October 23, 2009

13.5K
A Multiplex Serological Assay for the Detection of Antibody Responses to Arboviruses
05:22

A Multiplex Serological Assay for the Detection of Antibody Responses to Arboviruses

Published on: November 4, 2025

1.0K
Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles
08:50

Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

Published on: October 10, 2013

11.0K

Area of Science:

  • Food Science
  • Analytical Chemistry
  • Toxicology

Background:

  • Mycotoxins are fungal secondary metabolites that frequently contaminate food and feed, posing significant health risks to humans and animals.
  • Existing detection methods can be time-consuming and may not efficiently identify multiple contaminants simultaneously.
  • Rapid and accessible screening tools are crucial for ensuring the safety of food commodities.

Purpose of the Study:

  • To develop and validate a semi-quantitative multiplex immunoassay for the simultaneous detection of seven key mycotoxins.
  • To enable fast and easy screening of multi-contaminated food and feed commodities, particularly barley.
  • To provide a pre-screening tool that can help avoid costly instrumental analyses for samples below regulatory limits.

Main Methods:

  • Development of a 6-plex immunoassay using paramagnetic, color-coded microspheres and imaging planar array detection.
  • Antibody-based detection system utilizing mycotoxin-conjugated R-phycoerythrin as reporter molecules in a direct inhibition assay format.
  • Validation using blank and spiked barley samples, followed by screening of barley and malted barley reference materials.

Main Results:

  • The assay demonstrated high inter- and intra-day precision, with a maximum relative standard deviation of 10%.
  • The 6-plex immunoassay successfully detected aflatoxin B1, ochratoxin A, zearalenone, deoxynivalenol, T2-toxin, HT-2 toxin, and fumonisin B1 in barley samples.
  • Established screening cut-off levels aligned with EU legislation, enabling detection at specific low concentrations (e.g., 2 μg kg⁻¹ for aflatoxin B1).

Conclusions:

  • The developed semi-quantitative 6-plex immunoassay provides a rapid, easy-to-use, and precise method for multiplex mycotoxin screening in barley.
  • The portable system holds potential for on-site testing and serves as an effective pre-screening tool, complementing instrumental analysis.
  • This method supports compliance with EU regulations and enhances food safety by enabling efficient detection of multiple mycotoxin contaminants.