Related Experiment Videos
Bovine islets of Langerhans. Potential source for transplantation?
B J Hering1, D Romann, A Clarius
1Medical Clinic III and Policlinic, Justus Liebig University, Giessen, Federal Republic of Germany.
Abstract:
Two methods are described for large-scale isolation of islets from the bovine pancreas. With the single-endpoint technique the average number of islets isolated from a 73.3-g pancreas sample was 150,560, representing an estimated total islet volume of 0.391 ml. Pancreases processed by the continuous digestion-filtration method yielded an average of 246,229 islets from a 93.9-g pancreas sample, representing an estimated total islet volume of 0.458 ml. Isolated bovine pancreatic islets demonstrated a well-preserved endocrine cell ultrastructure, responded to acute stimulation with propionic acid, and temporarily reversed experimental diabetes in mice.