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Updated: Apr 28, 2026

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
Published on: July 11, 2020
Computational analysis, biochemical purification, and detection of tRNA-derived small RNA fragments
Simon P Keam1, Andrew Sobala, David T Humphreys
1Faculty of Engineering and Information Technology, Centre of Health Technologies, University of Technology, Sydney, 235 Jones Street, Ultimo, Sydney, NSW, 2007, Australia.
Abstract:
The rapidly growing list of small RNA species generated by next-generation sequencing technologies has accelerated the development of new bioinformatics tools for their detection. Small RNAs generated from tRNAs, transfer RNA-derived fragments (tRFs), represent a novel challenge in accurately identifying and distinguishing them from random degradation products of tRNAs. Here, we describe a bioinformatics approach to detect tRFs in next-generation sequencing libraries. We also present a biochemical purification protocol for enriching 5' tRFs and separating them from miRNAs. And finally, we suggest reliable methods for detecting and quantifying tRFs.
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