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N-glycoprotein surfaceomes of four developmentally distinct mouse cell types
Erin M Kropp1, Subarna Bhattacharya, Matthew Waas
1Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI, USA.
Proteomics. Clinical Applications
|June 13, 2014
Summary
This study identified over 600 cell surface N-glycoproteins during early mouse embryonic development. These findings offer new markers for cell identification and isolation in developmental studies.
Area of Science:
- Developmental Biology
- Proteomics
- Cell Biology
Background:
- Cell surface proteins are crucial for cell signaling and serve as markers for cell identification.
- Understanding these proteins during early embryogenesis is vital but currently limited for most cell lineages.
Purpose of the Study:
- To characterize the cell surface N-glycoproteome during early mouse embryonic development.
- To identify novel cell surface markers for distinct cell fates and developmental stages.
Main Methods:
- Utilized cell surface capture technology to analyze N-glycoproteomes.
- Assessed samples from three cell lines and one in vitro differentiated cell type representing key stages of mouse embryogenesis.
Main Results:
- Identified over 600 cell surface N-glycoproteins.
- Characterized more than 5500 N-glycopeptides.
Conclusions:
- The identified N-glycoproteins provide a foundation for developing new cell surface markers.
- These markers will facilitate the isolation of specific cell populations for developmental studies, disease modeling, and drug discovery.
