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Purification and characterization of the autolytic glycosidase of Streptococcus pneumoniae
P García1, J L García, E García
1Centro de Investigaciones Biológicas, C.S.I.C., Madrid, Spain.
Biochemical and Biophysical Research Communications
|January 16, 1989
Abstract:
A new lytic enzyme isolated from Streptococcus pneumoniae has been purified to electrophoretical homogeneity. The enzyme, showing a Mr of 64000, has been characterized as an endo-beta-1,4-N-acetylglucosaminidase that requires choline in the teichoic acid of the cell wall substrate for catalytic activity. In vivo experiments demonstrate that the glucosaminidase behaves as an autolytic enzyme.