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Updated: Apr 28, 2026

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Published on: March 3, 2015

Imaging intraorganellar Ca2+ at subcellular resolution using CEPIA.

Junji Suzuki¹, Kazunori Kanemaru¹, Kuniaki Ishii²

¹Department of Pharmacology, Graduate School of Medicine, The University of Tokyo, 7-3-1 Bunkyo-ku, Tokyo 113-0033, Japan.

Nature Communications

|June 14, 2014

Summary

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Researchers developed new fluorescent tools called calcium-measuring organelle-entrapped protein indicators (CEPIA) for imaging calcium (Ca2+) inside organelles. This allows detailed observation of calcium dynamics in the endoplasmic reticulum and mitochondria, revealing new insights into cellular functions.

Area of Science:

Cell Biology
Biochemistry
Molecular Imaging

Background:

Calcium ions (Ca2+) are crucial for regulating cellular functions, accumulating within the endoplasmic reticulum (ER) and mitochondria.
Directly measuring intraorganellar Ca2+ dynamics has been a significant challenge in cell biology.

Purpose of the Study:

To develop novel genetically encoded Ca2+ indicators for precise intraorganellar Ca2+ imaging.
To enable simultaneous analysis of Ca2+ dynamics across different cellular compartments.

Main Methods:

Engineered a family of fluorescent protein-based Ca2+ indicators (CEPIA) with organelle-targeting capabilities.
Utilized CEPIA for high spatiotemporal resolution imaging of Ca2+ in the ER, mitochondria, and cytosol.
Combined CEPIA imaging with other fluorescent markers for multi-functional analysis.

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Main Results:

Demonstrated CEPIA's ability to accurately bind Ca2+ at intraorganellar concentrations.
Achieved real-time visualization of Ca2+ import into individual mitochondria.
Revealed differential Ca2+ handling mechanisms within individual mitochondria.

Conclusions:

CEPIA provides a powerful new tool for studying organellar Ca2+ dynamics.
Enables unprecedented insights into the spatiotemporal regulation of Ca2+ in cellular processes.
Facilitates a deeper understanding of organelle function and intercellular communication.