Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peroxisomes01:24

Peroxisomes

13.6K
Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
13.6K
Peroxisomes01:24

Peroxisomes

1.8K
1.8K
Redox Reactions01:27

Redox Reactions

1.2K
Redox reactions are vital biochemical processes that underpin energy metabolism in cells. These reactions involve the transfer of electrons between molecules, occurring in tandem as oxidation and reduction. Oxidation refers to the loss of electrons, while reduction denotes their gain. This coupling ensures the seamless flow of electrons through metabolic pathways. For example, in bacterial metabolism, glucose undergoes oxidation to carbon dioxide, while oxygen is simultaneously reduced to...
1.2K
Redox Reactions01:24

Redox Reactions

50.9K
Oxidation-reduction or redox reactions involve the transfer of electrons from one molecule or atom to another. When an atom gains an electron, another atom must lose an electron, meaning oxidation and reduction must occur together. Since the redox occurs in pairs, the atom that gets oxidized is also called the reducing agent or reductant, and the atom that is reduced is also called the oxidizing agent or oxidant. A straightforward way to remember the definitions of oxidation and reduction is...
50.9K
Oxidation of Phenols to Quinones01:17

Oxidation of Phenols to Quinones

4.5K
In the presence of oxidizing agents, phenols are oxidized to quinones. Quinones can be easily reduced back to phenols using mild reducing agents. The electron-donating hydroxyl group enhances the reactivity of the aromatic ring, enabling oxidation of the ring even in the absence of an α hydrogen.
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox...
4.5K
Protein Import into the Peroxisomes01:27

Protein Import into the Peroxisomes

4.4K
Cells contain membrane-bound organelles called peroxisomes that oxidize organic molecules by transferring hydrogen atoms to oxygen, producing hydrogen peroxide. Peroxisomes enzymatically convert the released hydrogen peroxide into water and oxygen.
Peroxisomal Protein Import:
Peroxisomes lack the genetic machinery required to code for their own proteins. Hence, most peroxisomal membrane, lumenal and transmembrane proteins are synthesized in the cytoplasm or ER and transported to the peroxisome...
4.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lactoperoxidase is a candidate for mediating neuromelanin formation in the human substantia nigra.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Comparative analysis of naked mole-rat thermogenesis and its potential to maintain euthermia in response to cold.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Chemogenetic inhibition of the lateral hypothalamus effectively reduces food intake in rats in a translational proof-of-concept study.

Scientific reports·2024
Same author

KcsA-Kv1.x chimeras with complete ligand-binding sites provide improved predictivity for screening selective Kv1.x blockers.

The Journal of biological chemistry·2024
Same author

Autotaxin-lysophosphatidic acid receptor 5 axis evokes endothelial dysfunction via reactive oxygen species signaling.

Experimental biology and medicine (Maywood, N.J.)·2023
Same author

A novel monoclonal antibody reveals the enrichment of NADPH oxidase 5 in human splenic endothelial cells.

Scientific reports·2023

Related Experiment Video

Updated: Apr 28, 2026

Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ
10:05

Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ

Published on: May 8, 2020

1.8K

Peroxidasins: novel players in tissue genesis.

Zalán Péterfi1, Miklós Geiszt2

  • 1Department of Physiology, Faculty of Medicine, Semmelweis University, Budapest, H-1094, Hungary; Division of Genetics, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, 02115, USA.

Trends in Biochemical Sciences
|June 14, 2014
PubMed
Summary

Peroxidasin generates oxidants to form sulfilimine bonds in collagen IV, stabilizing the extracellular matrix. This protein crosslinking is crucial for tissue development and basal membrane structure.

Keywords:
ROScollagen IVextracellular matrixperoxidaseperoxidasinsulfilimine

More Related Videos

In Vivo Imaging of Reactive Oxygen Species in a Murine Wound Model
06:40

In Vivo Imaging of Reactive Oxygen Species in a Murine Wound Model

Published on: November 17, 2018

10.7K
Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry
10:24

Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry

Published on: June 7, 2018

8.1K

Related Experiment Videos

Last Updated: Apr 28, 2026

Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ
10:05

Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ

Published on: May 8, 2020

1.8K
In Vivo Imaging of Reactive Oxygen Species in a Murine Wound Model
06:40

In Vivo Imaging of Reactive Oxygen Species in a Murine Wound Model

Published on: November 17, 2018

10.7K
Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry
10:24

Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry

Published on: June 7, 2018

8.1K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Biophysics

Background:

  • Extracellular matrix (ECM) stabilization is vital for multicellular organisms.
  • Protein crosslinking plays a key role in ECM structure and function.
  • Peroxidasin, a heme-peroxidase, was recently implicated in ECM synthesis.

Purpose of the Study:

  • To elucidate the role of peroxidasin in ECM stabilization.
  • To identify the specific crosslinking mechanism mediated by peroxidasin.
  • To understand the significance of peroxidasin-dependent crosslinking in tissue biogenesis.

Main Methods:

  • Investigated peroxidasin activity in ECM stabilization.
  • Analyzed the chemical nature of crosslinks formed by peroxidasin.
  • Examined the impact of peroxidasin-dependent crosslinking on basal membrane structure.

Main Results:

  • Peroxidasin produces hypohalides, reactive oxidants essential for matrix synthesis.
  • These hypohalides unexpectedly mediate the formation of sulfilimine bonds between collagen IV protomers.
  • Sulfilimine crosslinks are a fundamental component of basal membranes.

Conclusions:

  • Peroxidasin-dependent oxidant generation and sulfilimine crosslink formation represent an elemental mechanism of tissue biogenesis.
  • This process is critical for the structural integrity and function of basal membranes.
  • The findings reveal a novel pathway for ECM stabilization with implications for developmental biology and regenerative medicine.