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Reduced recognition memory is correlated with decrease in DNA methyltransferase1 and increase in histone deacetylase2

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  • 1Biochemistry and Molecular Biology Laboratory, Department of Zoology, Brain Research Centre, Banaras Hindu University, Varanasi, 221 005, India.

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Aging reduces object recognition memory in mice. This decline correlates with decreased DNA methyltransferases (DNMTs) and increased histone deacetylase 2 (HDAC2) expression in the brain.

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Aging Research

Background:

  • Chromatin modifying enzymes, including DNA methyltransferases (DNMTs), histone deacetylase 2 (HDAC2), and CREB binding protein (CBP), are vital for memory consolidation.
  • Memory consolidation capacity diminishes with advancing age, but the underlying molecular mechanisms involving these enzymes remain unclear.

Purpose of the Study:

  • To investigate the age-related changes in the expression of DNMTs, HDAC2, and CBP.
  • To determine the correlation between the expression of these chromatin-modifying enzymes and memory consolidation during aging.

Main Methods:

  • Utilized the novel object recognition test to evaluate memory consolidation in young, adult, and old male mice.
  • Analyzed the expression levels of DNMTs, HDAC2, and CBP in the cerebral cortex and hippocampus.

Main Results:

  • Object recognition memory was significantly reduced in old mice compared to young and adult mice.
  • DNMT1 protein expression decreased with age in both the cerebral cortex and hippocampus.
  • HDAC2 mRNA and protein expression increased in the hippocampus of old mice.

Conclusions:

  • Age-related decline in memory consolidation is associated with altered expression of chromatin-modifying enzymes.
  • Reduced DNMT1 and increased HDAC2 expression may contribute to impaired memory in aging mice.