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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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Sanger Sequencing01:57

Sanger Sequencing

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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DNA Isolation01:24

DNA Isolation

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
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Chromosome Structure02:40

Chromosome Structure

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A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
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Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
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Library construction for ancient genomics: single strand or double strand?

E Andrew Bennett1, Diyendo Massilani1, Giulia Lizzo1

  • 1Institut Jacques Monod, CNRS, Université Paris Diderot, Paris, France.

Biotechniques
|June 14, 2014
PubMed
Summary
This summary is machine-generated.

The single-stranded DNA library method enhances ancient DNA recovery but is costly. Improvements make it more economical and versatile for various sequencing platforms, improving endogenous DNA yield.

Keywords:
DNA library preparationancient DNAnext-generation sequencingpalaeogenomics

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Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Ancient DNA (aDNA) research benefits from efficient library construction methods.
  • A novel single-stranded DNA ligase method shows promise for high-resolution ancient genomes.
  • Direct comparisons of various library construction methods across diverse aDNA samples are lacking.

Purpose of the Study:

  • To compare different DNA library construction methods for ancient samples.
  • To improve the cost-effectiveness and versatility of the single-stranded DNA method.
  • To optimize DNA purification for ancient samples.

Main Methods:

  • Comparative analysis of multiple library construction protocols.
  • Application to 16 diverse ancient and modern faunal and human DNA extracts.
  • Development of an improved DNA purification technique.
  • Methodological enhancements to the single-stranded DNA ligase method.

Main Results:

  • The single-stranded DNA library method generally improves endogenous DNA recovery from ancient samples.
  • The improved single-stranded method is more economical and compatible with Illumina and Ion Torrent platforms.
  • A new DNA purification method allows concentration of large volumes with minimal manipulation.

Conclusions:

  • The single-stranded DNA library construction method offers advantages for endogenous DNA recovery in ancient samples.
  • Methodological improvements enhance the practicality and accessibility of this technique.
  • Optimized purification and library construction expand possibilities for ancient DNA genomics.