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Primary structure of an N-linked sugar chain derived from glucoamylase of Rhizopus niveus
K Takegawa1, N Kawasaki, S Iwahara
1Department of Bioresource Science, Kagawa University, Japan.
Biochimica Et Biophysica Acta
|January 27, 1989
Abstract:
The primary structure of the N-linked sugar chain of Rhizopus niveus glucoamylase (major component) was investigated. The carbohydrate moiety was released from the polypeptide backbone by Flavobacterium sp. endo-beta-N-acetylglucosaminidase digestion. Studies using the method of exoglycosidase digestion of the fluorescent pyridylamino derivative, gel-permeation chromatography on Bio-Gel P-4 and 400-MHz 1H-NMR spectroscopy revealed that the most abundant structure is (Man)8-GlcNac-ol.