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Related Concept Videos

Protein Networks02:26

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An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
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Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
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Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
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Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay PCA in Living Cells
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Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay PCA in Living Cells

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The yeast protein interaction network has a capacity for self-organization.

Andi Dhroso1, Dmitry Korkin, Gavin C Conant

  • 1Department of Computer Science, University of Missouri, Columbia, MO, USA; Informatics Institute, University of Missouri, Columbia, MO, USA.

The FEBS Journal
|June 14, 2014
PubMed
Summary

Cellular organization arises from simple molecular interactions. Physical protein interactions create emergent enzyme micro-groups on protein clusters, revealing fundamental rules of cellular structure.

Keywords:
metabolic channelingprotein interaction networkrobustnessself-assembly

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Area of Science:

  • Cellular Biology
  • Biophysics
  • Systems Biology

Background:

  • Cellular interior organization enables metabolic channeling and signaling micro-compartmentalization.
  • Understanding molecular organization is key to cellular function.

Purpose of the Study:

  • To model molecular organization and stoichiometry in local cellular regions.
  • To investigate emergent structures from physical protein-protein interactions.

Main Methods:

  • Utilized a lattice model simulating molecular crowding.
  • Incorporated literature-derived protein interaction data and abundances.
  • Modeled protein interactions to observe emergent structures.

Main Results:

  • Physical protein-protein interactions induce emergent structures, unlike random networks.
  • Lattice models revealed micro-groups of enzymes on protein cluster surfaces.
  • Observed robustness to protein overexpression and evidence of expression tuning.

Conclusions:

  • Complex cellular organization may stem from simple molecular aggregation and interaction rules.
  • Emergent structures like enzyme micro-groups are a consequence of physical interactions.
  • Protein interactions play a crucial role in defining cellular architecture and function.