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Updated: Apr 28, 2026
Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling
Published on: August 3, 2018
[Effects of transforming growth factor-β1 gene silencing on the expression of vascular endothelial growth factor in
Yong Liao1, Mingxing Qiu1, Jing Liu1
1Department of Urology, Sichuan Academy of Medical Science & Sichuan Provincial People's Hospital, Chengdu 610072, China.
Objective:
To employ RNA interference technology to silence transforming growth factor-β1 (TGF-β1) gene to examine the gene silencing effects of RNAi on the expression of vascular endothelial growth factor (VEGF) in human bladder cancer cell lines (EJ).
Methods:
The TGF-β1 gene-specific siRNA expression vector was constructed. And the most efficiently suppressed target sequences were screened through reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). The samples were divided into 3 groups of EJ, control (TGF-β1) and recombinant plasmid (TGF-β1 siRNA expression vector). And the expression level of VEGF protein was detected by Western blot.
Results:
TGF-β1 gene-specific siRNA expression vector was constructed successfully. TGF-β1 relative mRNA expression was 0.92 ± 0.19 and the protein expression level (50 ± 6) pg/ml. The protein expression level of EJ group after transfection was (0.86 ± 0.18) pg/ml, control group (1.15 ± 0.29) pg/ml and recombinant plasmid group (0.45 ± 0.16) pg/ml(both P < 0.05).
Conclusions:
An inhibition of TGF-β1 gene down-regulates the expression of VEGF. And TGF-β1 may regulate angiogenesis of bladder tumor through an induction of VEGF gene expression.
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