Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

9.3K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.3K
Next-generation Sequencing03:00

Next-generation Sequencing

87.7K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
87.7K
Sanger Sequencing01:57

Sanger Sequencing

800.5K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
800.5K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

10.5K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
10.5K
Ribosome Profiling02:24

Ribosome Profiling

3.2K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Anxiety in post-covid-19 syndrome - prevalence, mechanisms and treatment.

Neuroscience applied·2025
Same author

Markers of Negative Emotionality in Individuals With Comorbid Alcohol Use Disorder and Post-Traumatic Stress Disorder: Role of Childhood Trauma.

Addiction biology·2025
Same author

Investigating non-inferiority of internet-delivered versus face-to-face cognitive behavioural therapy for insomnia (CBT-I): a randomised controlled trial (iSleep well).

Trials·2024
Same author

Pain in alcohol use disorder: Evaluating effects of childhood trauma, perceived stress, and psychological comorbidity.

Alcohol (Fayetteville, N.Y.)·2024
Same author

Clonal hematopoiesis related TET2 loss-of-function impedes IL1β-mediated epigenetic reprogramming in hematopoietic stem and progenitor cells.

Nature communications·2023
Same author

45 years German Society of Biological Psychiatry (DGBP).

Journal of neural transmission (Vienna, Austria : 1996)·2023
Same journal

Ketamine effects on EEG and their links to therapy differ across treatment-resistant major depression, post-traumatic stress disorder, and obsessive-compulsive disorder.

The international journal of neuropsychopharmacology·2026
Same journal

Genetic overlap between treatment-resistant schizophrenia and smoking initiation.

The international journal of neuropsychopharmacology·2026
Same journal

External Validation as the Critical Step for Predictive Biomarkers.

The international journal of neuropsychopharmacology·2026
Same journal

Viloxazine occupies the 5-HT2C receptor in the macaca fascicularis brain in vivo: A [11C]CIMBI-36 positron emission tomography study.

The international journal of neuropsychopharmacology·2026
Same journal

Divergent effects of interleukin-6 and ghrelin on remission and relapse in depression treatment.

The international journal of neuropsychopharmacology·2026
Same journal

Secondary Restless Legs Syndrome during psychopharmacological treatment: real-world evidence from a multinational pharmacovigilance program.

The international journal of neuropsychopharmacology·2026
See all related articles

Related Experiment Video

Updated: Apr 28, 2026

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

24.9K

Making sense of deep sequencing.

D Goldman1, K Domschke2

  • 1Laboratory of Neurogenetics, NIAAA, NIH, Rockville, MD,USA.

The International Journal of Neuropsychopharmacology
|June 14, 2014
PubMed
Summary
This summary is machine-generated.

Deep sequencing, also known as next-generation sequencing, offers powerful insights into DNA and RNA. This technology aids in identifying genetic diseases, understanding gene expression, and revealing environmental impacts, while also presenting ethical considerations.

More Related Videos

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

15.2K
Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples
11:23

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples

Published on: December 22, 2014

34.7K

Related Experiment Videos

Last Updated: Apr 28, 2026

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

24.9K
Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

15.2K
Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples
11:23

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples

Published on: December 22, 2014

34.7K

Area of Science:

  • Genomics and Molecular Biology
  • Bioinformatics
  • Epigenetics

Background:

  • Deep sequencing, encompassing next-generation, high-throughput, and massively parallel sequencing, analyzes polynucleic acids (DNA and RNA).
  • It extends beyond whole genome sequencing to target specific genomic regions like the exome or epigenetically modified DNA.
  • RNA sequencing (RNASeq) is crucial for assessing RNA expression and structural variations.

Purpose of the Study:

  • To elucidate the fundamental concepts and diverse applications of deep sequencing technologies.
  • To highlight key considerations in deep sequencing, including read length, depth, mapping, assembly, and error analysis.
  • To discuss the implications of deep sequencing in identifying genetic predispositions, environmental exposures, and associated ethical challenges.

Main Methods:

  • Review of current literature and conceptual frameworks surrounding deep sequencing.
  • Explanation of methodologies for analyzing DNA and RNA at high resolution.
  • Discussion of data processing, error correction, and validation strategies in deep sequencing.

Main Results:

  • Deep sequencing provides a detailed genetic fingerprint for individual identification and disease prediction.
  • RNASeq enables precise measurement of gene expression and detection of RNA structural alterations.
  • Epigenetic analysis via deep sequencing reveals environmental influences on DNA methylation and protein binding.

Conclusions:

  • Deep sequencing is a transformative tool in biomedical research and clinical practice.
  • It offers unprecedented insights into genetic makeup, disease risk, and environmental interactions.
  • The ethical and practical challenges of handling sensitive genetic information require careful consideration and robust protocols.