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Mammalian Cell Encapsulation in Alginate Beads Using a Simple Stirred Vessel
Published on: June 29, 2017
Janus-compartmental alginate microbeads having polydiacetylene liposomes and magnetic nanoparticles for visual
Do Hyun Kang1, Ho-Sup Jung, Namyoung Ahn
1Department of Mechanical and Aerospace Engineering, WCU Program for Multiscale Mechanical Design, ‡Institute of Advanced Machinery and Design, Department of Mechanical and Aerospace Engineering, §Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute for Agriculture and Life Sciences, Seoul National University , Seoul, 151-742, Republic of Korea.
Abstract:
Janus-compartmental alginate microbeads having two divided phases of sensory polydiacetylene (PDA) liposomes and magnetic nanoparticles were fabricated for facile sensory applications. The sensory liposomes are composed of PDA for label-free signal generation and 1,2-dipalmitoyl-sn-glycero-3-galloyl (DPGG) lipids whose galloyl headgroup has specific interactions with lead(II). The second phase having magnetic nanoparticles is designed for convenient handling of the microbeads, such as washing, solvent exchange, stirring, and detection, by applying magnetic field. Selective and convenient colorimetric detection of lead(II) and efficient removal of lead(II) by alginate matrix at the same time are demonstrated.
