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Beneficial effects of phlorizin on diabetic nephropathy in diabetic db/db mice
Fei Pei1, Bao-Ying Li2, Zhen Zhang2
1Department of Nephrology, Qi-Lu Hospital of Shandong University, Shandong Province 250012, People's Republic of China.
Aims:
This study observes the effects of phlorizin on diabetic nephrology in db/db diabetic mice and explores possible underlying mechanisms.
Methods:
Sixteen diabetic db/db mice and eight age-matched db/m mice were divided into three groups: vehicle-treated diabetic group (DM group), diabetic group treated with phlorizin (DMT group) and normal control group (CC group). Phlorizin was given in normal saline solution by intragastric administration for 10 weeks. Differentially expressed proteins in three groups were identified using iTRAQ quantitative proteomics and the data were further analyzed with ingenuity pathway analysis.
Results:
The body weight and serum concentrations of fasting blood glucose (FBG), advanced glycation end products (AGEs), total cholesterol, triglycerides, blood urea nitrogen, creatinine and 24-h urine albumin were increased in the DM group compared to those of the CC group (P<0.05), and they were decreased by treatment with phlorizin (P<0.05). Morphologic observations showed phlorizin markedly attenuated renal injury. Phlorizin prevented diabetic nephropathy by regulating the expression of a series of proteins involved in renal and urological disease, molecular transport, free radical scavenging, and lipid metabolism.
Conclusions:
Phlorizin protects mice from diabetic nephrology and thus may be a novel therapeutic approach for the treatment of diabetic nephrology.
Insights
Phlorizin treatment significantly reduced markers of kidney damage in diabetic mice, suggesting its potential as a novel therapy for diabetic nephropathy.
Area of Science:
- Nephrology
- Endocrinology
- Proteomics
Background:
- Diabetic nephropathy is a major complication of diabetes.
- Understanding its mechanisms is crucial for developing effective treatments.
Purpose of the Study:
- To investigate the protective effects of phlorizin on diabetic nephropathy in db/db mice.
- To explore the underlying molecular mechanisms of phlorizin's action.
Main Methods:
- db/db diabetic mice and db/m control mice were treated with phlorizin or vehicle for 10 weeks.
- iTRAQ quantitative proteomics identified differentially expressed proteins.
- Ingenuity pathway analysis elucidated molecular pathways involved.
Main Results:
- Phlorizin treatment attenuated increases in body weight, blood glucose, lipids, and kidney injury markers.
- Histological analysis confirmed reduced renal injury in phlorizin-treated mice.
- Phlorizin modulated protein expression related to renal disease, transport, and metabolism.
Conclusions:
- Phlorizin demonstrates protective effects against diabetic nephropathy in mice.
- Phlorizin may represent a promising therapeutic strategy for diabetic kidney disease.
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