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LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles
Published on: February 1, 2020
[Relationship between Line 1 methylation and clinical data of non-small cell lung cancer]
Xinneng Liu1, Yongtang Jin, Peiwei Xu
1Environmental Epigenetics Laboratory, Department of Environmental Medicine, Faculty of Public Health, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310058, P.R. China. Emailjinedu@zju.edu.cn.
Objective:
To explore the impact of Line-1 methylation on clinical features of non-small cell lung cancer and its connection with smoking and other living habits.
Methods:
Pyrosequencing was used to determine the extent of Line-1 methylation in cancer and adjacent tissues derived from 197 patients with primary non-small cell lung cancer. Non-conditional logistic regression analysis was performed to correlate the level of Line-1 methylation with clinical features and living habits of the patients.
Results:
Line-1 methylation for cancer tissue and adjacent tissue has measured 68.20±11.63 and 78.90±2.09, respectively (P < 0.01), and has been associated with TNM staging, smoking history and histopathological types.
Conclusion:
Lung cancer tissue Line-1 methylation level is closely related with clinical features and smoking. There is also a correlation between histopathological types of lung cancer and relative hypomethylation of Line-1.
