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Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
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Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

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Fission yeast mtr1p regulates interphase microtubule cortical dwell-time

Frédérique Carlier-Grynkorn1, Liang Ji1, Vincent Fraisier1

  • 1Institut Curie, Paris 75005, France CNRS, UMR 144, Paris 75005, France.

Biology Open
|June 15, 2014
PubMed

Insights

Fission yeast mtr1 deletion results in longer microtubule dwell-time, suggesting mtr1p destabilizes microtubules. This novel regulator may involve the ribosome complex in microtubule dynamics.

Area of Science:

  • Cell Biology
  • Cytoskeleton Dynamics
  • Molecular Genetics

Background:

  • Microtubules are crucial for cell functions like division and motility.
  • Microtubule dynamics are regulated by microtubule-associated proteins (MAPs) and motors.
  • Understanding novel regulators of microtubule dynamics is essential for cell biology.

Purpose of the Study:

  • To identify and characterize new regulators of microtubule dynamics in fission yeast.
  • To investigate the role of the novel protein mtr1p in microtubule stability.
  • To explore the potential connection between mtr1p, ribosome function, and microtubule regulation.

Main Methods:

  • Genetic analysis of fission yeast mutants, including mtr1 deletion (mtr1Δ) and mal3 deletion (mal3Δ).
  • Microscopy to observe and quantify microtubule behavior and dwell-time.
  • Sequence homology analysis to predict protein function and cellular localization.

Main Results:

  • mtr1Δ mutants exhibit significantly longer microtubule dwell-times, indicating mtr1p acts as a microtubule destabilizer.
  • mtr1p function is antagonistic to mal3p (EB1 ortholog), a known microtubule stabilizer.
  • Deletion of a predicted ribosomal gene (rps1801) also increased microtubule dwell-time, similar to mtr1Δ.
  • Double mutants (mal3Δ mtr1Δ and mal3Δ rps1801Δ) show partially rescued microtubule lengths compared to mal3Δ single mutants.

Conclusions:

  • Fission yeast mtr1p is a novel regulator of microtubule dynamics, acting as a destabilizer.
  • mtr1p's function may be linked to the ribosome quality control complex.
  • This study suggests a potential role for the ribosome complex in modulating microtubule dynamics.
Keywords:
Dynamic instabilityMicrotubuleRibosome

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