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Increased internalization of Staphylococcus aureus and cytokine expression in human Wharton's jelly mesenchymal stem
Jérôme Josse1, Frédéric Velard2, Saad Mechiche Alami3
1EA 4691 «Biomatériaux et Inflammation en Site Osseux», Pôle Santé, SFR CAP-Santé (FED 4231), Université de Reims Champagne-Ardenne, Reims, France UFR de Pharmacie, URCA, Reims, France.
Background:
Although a large number of studies have documented the interaction of mesenchymal stem cells (MSCs) with cells of both the innate and adaptive immune systems, not much is known about how bacteria interact with MSCs and how this might influence MSCs behavior. In this study, we investigated the impact of Staphylococcus aureus (S. aureus), on viability and cytokines' production of human Wharton's jelly-MSCs (WJ-MSCs).
Objective:
To investigate if WJ-MSCs: (1) internalize S. aureus; (2) are able to survive and (3) release immunomodulatory mediators after interaction with S. aureus.
Methods:
WJ-MSCs were exposed to S. aureus at a multiplicity of infection (MOI) of 10:1 or 30:1 for different designed times. After interaction, intracellular bacteria were quantified as well as MSCs viability. Expression and cytokine-secretion were assessed using quantitative real-time PCR and ELISA.
Results:
We found that the challenge of WJ-MSCs with S. aureus resulted in increased internalization of S. aureus in a time-dependent manner until six hours post-infection at either MOI of 10:1 and 30:1 and in increased expression of IL-6 mRNA and secretion of TNF-α at six hours and nine hours post-infection (p<0.05).
Conclusions:
These results indicate that WJ-MSCs are able to internalize S. aureus and reveal a potential important function of these cells in the immune response.
Insights
Human Wharton's jelly-mesenchymal stem cells (WJ-MSCs) internalize Staphylococcus aureus (S. aureus), increasing IL-6 and TNF-α production. This suggests WJ-MSCs play a role in the immune response to bacterial infections.
Area of Science:
- Immunology
- Stem Cell Biology
- Microbiology
Background:
- Mesenchymal stem cells (MSCs) interact with immune cells, but bacterial interactions with MSCs are less understood.
- This study explores the impact of Staphylococcus aureus (S. aureus) on human Wharton's jelly-MSCs (WJ-MSCs).
Purpose of the Study:
- To determine if WJ-MSCs internalize S. aureus.
- To assess WJ-MSC survival after S. aureus exposure.
- To investigate the release of immunomodulatory mediators by WJ-MSCs following S. aureus interaction.
Main Methods:
- WJ-MSCs were exposed to S. aureus at multiplicities of infection (MOI) of 10:1 and 30:1.
- Intracellular S. aureus and WJ-MSC viability were quantified.
- Cytokine expression (IL-6 mRNA) and secretion (TNF-α) were measured using qRT-PCR and ELISA.
Main Results:
- WJ-MSCs internalized S. aureus in a time-dependent manner up to six hours post-infection.
- Increased expression of IL-6 mRNA and secretion of TNF-α were observed at six and nine hours post-infection.
- No significant impact on WJ-MSC viability was explicitly stated in the results.
Conclusions:
- WJ-MSCs demonstrate the capacity to internalize S. aureus.
- These findings highlight a potential role for WJ-MSCs in the immune response against bacterial pathogens.
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