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Updated: Apr 28, 2026
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Extraction of High Molecular Weight Genomic DNA from Soils and Sediments
Published on: November 10, 2009
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A sequential co-extraction method for DNA, RNA and protein recovery from soil for future system-based approaches
Eoin Gunnigle1, Jean-Baptiste Ramond1, Aline Frossard1
1Centre for Microbial Ecology and Genomics (CMEG), Department of Genetics, University of Pretoria, South Africa.
Journal of Microbiological Methods
|June 15, 2014
Abstract:
A co-extraction protocol that sequentially isolates core biopolymer fractions (DNA, RNA, protein) from edaphic microbial communities is presented. In order to confirm compatibility with downstream analyses, bacterial T-RFLP profiles were generated from the DNA- and RNA-derived fractions of an arid-based soil, with metaproteomics undertaken on the corresponding protein fraction.
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