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Related Concept Videos

Indicators02:39

Indicators

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Certain organic substances change color in dilute solution when the hydronium ion concentration reaches a particular value. For example, phenolphthalein is a colorless substance in any aqueous solution with a hydronium ion concentration greater than 5.0 × 10−9 M (pH < 8.3). In more basic solutions where the hydronium ion concentration is less than 5.0 × 10−9 M (pH > 8.3), it is red or pink. Substances such as phenolphthalein, which can be used to determine the pH...
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Precipitation Titration: Endpoint Detection Methods01:19

Precipitation Titration: Endpoint Detection Methods

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In argentometric precipitation titrations, endpoints can be detected visually by the Mohr, Volhard, and Fajans methods. In the Mohr method, adding a soluble chromate indicator gives an initial yellow color to the analyte solution. As the titrant is added, the first excess of silver ions forms a red silver chromate precipitate, marking the endpoint. The solution pH should be maintained at about 8 by adding solid CaCO3.
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Titration of a Weak Acid with a Weak Base01:08

Titration of a Weak Acid with a Weak Base

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Weak acids and bases do not undergo dissociation completely, and titrations between these two are rarely studied. When such studies are performed, say, for the titration of a weak acid with a weak base, the titration curve plots the change in pH as a function of the volume of base added. Take the titration of acetic acid with ammonia, for instance. During the titration, these two species form ammonium acetate and water, but the pH change is slow and gradual.
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Quantitative Analysis01:12

Quantitative Analysis

1.7K
Quantitative analysis is a technique for measuring the amount of specific constituents in a sample. When the sample's composition is unknown, qualitative analysis is performed first to identify its components, which ensures that the correct substances are measured during the quantitative phase.
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Glassware Calibration01:11

Glassware Calibration

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Accurate calibration of glassware, such as volumetric flasks, pipettes, and burettes, is essential to ensure accurate measurements in the analytical laboratory. Calibration helps maintain consistency across measurements and prevents errors arising from inaccurate volumes.
Volumetric flasks: Volumetric flasks are designed to prepare aqueous solutions of precise volumes accurately with a calibration line on the neck. To calibrate a volumetric flask, it is important to fill it with distilled...
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Bergmeyer Glucose Quantification for Microbiological Samples
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Quantitative pH assessment of small-volume samples using a universal pH indicator.

Jeffrey D Brown1, Nathaniel Bell1, Victoria Li2

  • 1Department of Biology, University of Portland, Portland, OR 97203, USA.

Analytical Biochemistry
|June 15, 2014
PubMed
Summary

We created a simple, image-based method to measure pH in small liquid samples using a universal pH indicator. This technique offers accurate pH determination for various applications without needing complex calibration.

Keywords:
Isoelectric focusingWntpH determinationpH indicator

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Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Biochemistry

Background:

  • Accurate pH measurement is crucial in biological and chemical analyses.
  • Traditional methods can be challenging for small-volume samples or high-throughput screening.

Purpose of the Study:

  • To develop a novel, accessible hue-based method for determining sample pH using digital imaging.
  • To validate the method's accuracy and precision for various pH standards and biological samples.

Main Methods:

  • A hue-based colorimetric approach was employed to analyze digital images of samples treated with a universal pH indicator.
  • Digital images of samples in a 384-well plate were processed to determine pH based on color hue.
  • Isoelectric focusing was used to determine the isoelectric point of Wnt3A protein for sample pH assessment.

Main Results:

  • The developed method achieved a standard error of calibration of 0.078 pH units across 69 pH standards.
  • Individual sample pH measurements exhibited a maximum error of 0.23 pH units.
  • The method was successfully applied to assess the pH of small-volume Wnt3A protein samples.

Conclusions:

  • The hue-based pH determination method provides a reliable and accurate tool for analyzing small-volume samples.
  • The method simplifies pH assessment, offering a user-friendly standard for researchers without requiring additional calibration.
  • This technique has potential applications in various fields requiring precise pH monitoring of limited sample volumes.