Related Experiment Video
Updated: Apr 28, 2026
Two Methods for Decellularization of Plant Tissues for Tissue Engineering Applications
Published on: May 31, 2018
Morphofunctional characterization of decellularized vena cava as tissue engineering scaffolds.
Matheus Bertanha1, Andrei Moroz2, Rodrigo G Jaldin3
1Department of Surgery and Orthopedics, Botucatu Medical School, São Paulo State University (UNESP), Vascular Laboratory, Avenue Prof. Montenegro, S/N. Rubião Júnior, Botucatu 18618-970, SP, Brazil; Blood Transfusion Center, Cell Engineering Laboratory, Botucatu Medical School, São Paulo State University (UNESP), Botucatu, SP, Brazil.
Sodium dodecyl sulfate (SDS) and sodium deoxycholate (DS) effectively decellularized rabbit vena cava scaffolds, preserving extracellular matrix integrity and biomechanical properties for potential use in vascular tissue engineering.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Vascular Surgery
Background:
- Synthetic grafts show poor outcomes for infrapopliteal peripheral arterial disease treatment.
- Tissue engineering offers a promising alternative for creating biocompatible neovessels.
- Decellularized biological scaffolds can provide natural tissue architecture when autologous veins are unavailable.
Purpose of the Study:
- To evaluate chemical-induced decellularization protocols for rabbit inferior vena cava.
- To assess the efficacy of Triton X100 (TX100), sodium dodecyl sulfate (SDS), and sodium deoxycholate (DS) in decellularization.
- To characterize the resulting scaffolds for extracellular matrix (ECM) integrity, residual toxicity, and biomechanical resistance.
Main Methods:
- Rabbit inferior vena cava were decellularized using TX100, SDS (1%), or DS (2%).
- Scaffolds were analyzed for cell removal efficiency and ECM integrity.
- Residual toxicity and biomechanical properties were compared to native tissue.
Main Results:
- TX100 was ineffective for cell removal.
- SDS (1% for 2h) and DS (2% for 1h) protocols efficiently removed cells while preserving ECM organization.
- Decellularized scaffolds showed no significant residual toxicity and retained biomechanical properties comparable to the control.
Conclusions:
- SDS and DS-based protocols are effective for decellularizing rabbit vena cava.
- These decellularized scaffolds maintain structural and mechanical integrity.
- The developed scaffolds show potential for vascular tissue engineering applications.
