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Sodium thiosulfate inhibits cisplatin-induced mutagenesis in somatic tissue of Drosophila
1Department of Biological Sciences, Illinois State University, Normal 61761.
Abstract:
The antitumor agent cisplatin was evaluated for genotoxicity in the somatic tissue of Drosophila melanogaster in combination with the nucleophilic compound sodium thiosulfate (STS). Third instar larvae transheterozygous for mwh and flr3 were grown on media containing (1) water, (2) 200 mM STS, (3) 0.05 mM cisplatin in 200 mM STS, or (4) 0.05 mM cisplatin. Wings of surviving adults were scored for the presence of twin spots and both small and large single spots; 200 mM STS was nongenotoxic in the assay. Whereas 0.05 mM cisplatin significantly induced all three endpoints, 0.05 mM cisplatin in combination with 200 mM STS was found to have no genotoxic activity. Hence, STS possessed antimutagenic activity in the wing-spot assay and completely inhibited cisplatin-induced mutagenesis and mitotic recombination.
Insights
Sodium thiosulfate (STS) demonstrated antimutagenic properties by completely inhibiting genotoxicity and mutagenesis induced by the antitumor agent cisplatin in Drosophila melanogaster wing-spot assays.
Area of Science:
- Genetics
- Toxicology
- Developmental Biology
Background:
- Cisplatin is a widely used chemotherapy drug with known genotoxic side effects.
- Investigating protective agents against genotoxicity is crucial for improving cancer therapy.
- Drosophila melanogaster serves as a valuable model organism for genotoxicity studies.
Purpose of the Study:
- To evaluate the genotoxicity of cisplatin.
- To assess the potential antimutagenic effect of sodium thiosulfate (STS) against cisplatin-induced genotoxicity.
- To investigate the interaction between cisplatin and STS in a living organism model.
Main Methods:
- The study utilized the Drosophila melanogaster wing-spot assay to assess genotoxicity.
- Third instar larvae were exposed to cisplatin, sodium thiosulfate (STS), or a combination of both.
- Wing spots (twin spots, small single spots, large single spots) were quantified in surviving adults.
Main Results:
- Sodium thiosulfate (STS) alone was found to be non-genotoxic.
- Cisplatin significantly induced genotoxicity, as evidenced by increased wing spots.
- The combination of cisplatin and STS showed no genotoxic activity, indicating STS's protective effect.
Conclusions:
- Sodium thiosulfate (STS) exhibits significant antimutagenic activity.
- STS effectively inhibits cisplatin-induced mutagenesis and mitotic recombination in Drosophila melanogaster.
- STS holds potential as a protective agent against cisplatin-induced genotoxicity.