Related Experiment Video

Updated: Apr 28, 2026

Characterization of MLKL-mediated Plasma Membrane Rupture in Necroptosis
08:55

Characterization of MLKL-mediated Plasma Membrane Rupture in Necroptosis

Published on: August 7, 2018

10.1K

[The molecular mechanism of necroptosis]

Ba Wei1, Pang Yue1, Li Qingwei1

  • 1College of Life Science, Liaoning Normal University, Dalian 116029, China; Lamprey Research Center, Liaoning Normal University, Dalian 116029, China.

Yi Chuan = Hereditas
|June 16, 2014
PubMed

Insights

Necroptosis, a programmed necrosis distinct from apoptosis, is initiated by factors like TNFR or PRR, involving key proteins RIP1 and RIP3. This cell death pathway releases damage-associated molecular patterns (DAMPs) for clearance.

Area of Science:

  • Cellular Biology
  • Immunology
  • Molecular Biology

Context:

  • Necroptosis is a regulated form of necrosis distinct from apoptosis.
  • It plays a role in inflammation and immunity.
  • Recent research highlights its significance in various biological processes.

Purpose:

  • To review the key molecular players and pathways involved in necroptosis.
  • To discuss the initiation, execution, and downstream consequences of necroptosis.
  • To highlight the process of damage-associated molecular pattern (DAMP) release and clearance.

Summary:

  • Necroptosis is triggered by stimuli such as tumor necrosis factor receptor (TNFR) and pattern recognition receptors (PRR), with receptor-interacting protein (RIP) 1 and 3 as crucial mediators.
  • Key molecules like PARP-1, ROS, and Ca(2+) contribute to organelle damage and caspase-independent cell death.
  • Released DAMPs are subsequently recognized and cleared by phagocytes, ensuring tissue homeostasis.

Impact:

  • Understanding necroptosis provides insights into inflammatory diseases and cancer.
  • Modulators of necroptosis may represent therapeutic targets.
  • Elucidating DAMP release and clearance mechanisms is vital for immune regulation.

More Related Videos

Live-cell Imaging of Lysosomal Membrane Permeabilization During Necroptosis05:30

Live-cell Imaging of Lysosomal Membrane Permeabilization During Necroptosis

Published on: November 14, 2025

603
Author Spotlight: THP-1 Macrophage Response to LPS/ATP — Unveiling the Pyroptosis, Apoptosis, and Necroptosis Spectrum06:12

Author Spotlight: THP-1 Macrophage Response to LPS/ATP — Unveiling the Pyroptosis, Apoptosis, and Necroptosis Spectrum

Published on: May 3, 2024

3.4K

Related Concept Videos

Necrosis01:16

Necrosis

Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”.
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...
5.2K
Overview of Cell Death01:30

Overview of Cell Death

Cell death is an essential process where the body gets rid of old or damaged cells. Cell proliferation and death need to be balanced, as an imbalance between the two may lead to cancer or autoimmune diseases.
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the...
7.6K
The Extrinsic Apoptotic Pathway01:17

The Extrinsic Apoptotic Pathway

The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
6.1K
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
6.2K
Autophagic Cell Death01:18

Autophagic Cell Death

Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
3.3K
Cellular Injury IV: Necrosis01:16

Cellular Injury IV: Necrosis

Necrosis is a form of irreversible cell death caused by severe injury such as ischemia, toxins, or trauma. Unlike programmed cell death, it is an uncontrolled, pathological process that typically provokes inflammation in surrounding tissues.Pathophysiologic ChangesNecrosis begins when cells sustain critical damage, leading to swelling of organelles, particularly mitochondria, and rapid ATP depletion. As energy levels decline, membrane ion pumps fail, leading to calcium influx and eventually,...
60