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Screening Foodstuffs for Class 1 Integrons and Gene Cassettes
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Screening Foodstuffs for Class 1 Integrons and Gene Cassettes

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[Structure and function of class 1 integron in clinical isolates of Klebsiella pneumoniae]

Zhang Yuejin1, Chang Qingli2, Wang Qian2

  • 1School of Laboratory and Life Sciences, Wenzhou Medical University, Wenzhou 325000, China; School of Nursing, Wenzhou Medical University, Wenzhou 325000, China;

Yi Chuan = Hereditas
|June 16, 2014
PubMed

Insights

Class 1 integrons are common in Klebsiella pneumoniae, contributing to multi-drug resistance. These integrons carry genes conferring resistance to aminoglycosides and sulfonamides, facilitating spread through transferable plasmids.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Klebsiella pneumoniae exhibits significant multi-drug resistance.
  • Investigating the molecular mechanisms of resistance is crucial for understanding its spread.

Purpose of the Study:

  • To investigate the molecular mechanisms behind multi-drug resistance in Klebsiella pneumoniae.
  • To determine the role of class 1 integrons in the dissemination of antibiotic resistance.

Main Methods:

  • Analysis of 179 Klebsiella pneumoniae strains isolated between 2002-2007.
  • Detection and characterization of class 1 integrons and their gene cassettes.
  • Cloning of resistance genes and assessment of antibiotic resistance activity in E. coli.

Main Results:

  • 65.9% of strains harbored class 1 integrons, significantly associated with resistance to aminoglycosides, quinolones, and sulfonamides.
  • Fifteen gene cassettes were identified, with dfrA17-aadA5 being most prevalent.
  • Recombinant plasmids conferred resistance to co-trimoxazole and streptomycin, with one plasmid showing high-level streptomycin resistance.

Conclusions:

  • Class 1 integrons are frequently found in Klebsiella pneumoniae and carry key resistance genes.
  • Transferable plasmids containing integrons contribute significantly to the spread of antibiotic resistance among bacterial species.

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