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Quantifying Glosair™ 400 efficacy for surface disinfection of American Type Culture Collection strains and
R Herruzo1, M J Vizcaíno1, I Herruzo2
1Department of Preventive Medicine, Public Health and Microbiology, School of Medicine, Universidad Autónoma de Madrid, Madrid, Spain.
Abstract:
Microbial contamination of hospital surfaces may be a source of infection for hospitalized patients. We evaluated the efficacy of Glosair™ 400 against two American Type Culture Collection strains and 18 clinical isolates, placed on glass germ-carriers. Carriers were left to air-dry for 60 min and then exposed to a cycle before detection of any surviving micro-organisms. Antibiotic-susceptible Gram-negative bacilli were less susceptible (although not significantly) to this technique than resistant Gram-negative bacilli or Gram-positive cocci and yeasts (3, 3.4 and 4.6 log10 reduction, respectively). In conclusion, in areas that had not been cleaned, aerosolized hydrogen peroxide obtained >3 log10 mean destruction of patients' micro-organisms.
Insights
Aerosolized hydrogen peroxide effectively reduced microbial contamination on hospital surfaces, achieving over a 3-log10 reduction of patient microorganisms in uncleaned areas. This disinfection method shows promise for enhancing hospital hygiene and patient safety.
Area of Science:
- Environmental microbiology
- Hospital infection control
- Antimicrobial efficacy
Background:
- Hospital-acquired infections pose significant risks to patients.
- Contaminated surfaces are a potential reservoir for pathogenic microorganisms.
- Effective surface disinfection is crucial for preventing infection transmission.
Purpose of the Study:
- To evaluate the antimicrobial efficacy of Glosair™ 400 (aerosolized hydrogen peroxide).
- To assess the reduction of various microbial strains on contaminated surfaces.
- To determine the effectiveness of this disinfection method in a simulated hospital environment.
Main Methods:
- Testing Glosair™ 400 against American Type Culture Collection strains and clinical isolates.
- Contaminating glass germ-carriers with microorganisms and allowing them to air-dry.
- Exposing contaminated carriers to an aerosolized hydrogen peroxide cycle and quantifying surviving microorganisms.
Main Results:
- A mean reduction of >3 log10 of patient microorganisms was observed in uncleaned areas.
- Gram-negative bacilli showed varying susceptibility, with antibiotic-susceptible strains being less susceptible than resistant strains.
- Gram-positive cocci and yeasts demonstrated higher susceptibility, with 4.6 log10 reduction.
Conclusions:
- Aerosolized hydrogen peroxide demonstrates significant antimicrobial activity against a range of hospital-relevant microorganisms.
- The disinfection technique is effective in reducing microbial load on surfaces.
- This method offers a potential strategy for improving environmental hygiene in healthcare settings.