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Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
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Microfluidic Channels on Nanopatterned Substrates: Monitoring Protein Binding to Lipid Bilayers with Surface-Enhanced

Amrita Banerjee1, R Perez-Castillejos2, D Hahn3

  • 1Electronic Imaging Center, New Jersey Institute of Technology, Newark, New Jersey, 07102.

Chemical Physics Letters
|June 17, 2014
PubMed
Summary

Surface Enhanced Raman Spectroscopy (SERS) detected streptavidin-biotin binding on anodized aluminum oxide substrates within microfluidic channels. This method effectively distinguishes bound molecules from unbound ones and minimizes interference from large cells.

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Area of Science:

  • Biomolecular Interaction Analysis
  • Surface Science
  • Analytical Chemistry

Background:

  • Studying molecular binding is crucial for biosensor development.
  • Microfluidic systems offer controlled environments for surface interactions.
  • Anodized aluminum oxide (AAO) provides a versatile substrate for surface functionalization.

Purpose of the Study:

  • To detect and characterize binding events between streptavidin and biotinylated lipid bilayers.
  • To evaluate the efficacy of Surface Enhanced Raman Spectroscopy (SERS) for this detection.
  • To assess the influence of microfluidic integration and substrate choice on binding detection.

Main Methods:

  • Utilized Surface Enhanced Raman Spectroscopy (SERS) for sensitive molecular detection.
  • Employed microfluidic channels integrated with anodized aluminum oxide (AAO) substrates.
  • Incorporated biotinylated lipid bilayers onto the AAO substrates for streptavidin binding.

Main Results:

  • Successfully detected specific binding events between streptavidin and biotinylated lipid bilayers.
  • Demonstrated that unbound molecules are efficiently removed through washing.
  • Showed that large suspended cells, like *Salmonella enterica*, cause minimal interference when monitoring the lower channel surface.

Conclusions:

  • SERS is a viable technique for monitoring biomolecular binding in microfluidic systems.
  • AAO substrates are suitable for immobilizing lipid bilayers and detecting binding events.
  • The SERS method allows for selective detection of bound molecules, even in the presence of potential interferents.