Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transgenic Plants02:50

Transgenic Plants

7.0K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.0K
Reproductive Cloning01:27

Reproductive Cloning

28.4K
Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic...
28.4K
Reproductive Cloning01:27

Reproductive Cloning

2.7K
2.7K
Plant Tissue Culture02:57

Plant Tissue Culture

33.3K
Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.
33.3K
Plant Breeding and Biotechnology01:59

Plant Breeding and Biotechnology

17.2K
Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
17.2K
Cloning of Dolly the Sheep01:08

Cloning of Dolly the Sheep

8.7K
The first successfully cloned mammal was Dolly, a sheep, born on 5th July 1996 at Roslin Institute, Scotland. The cloned sheep was named after the American singer Dolly Parton. Dolly lived for seven years and died of respiratory complications, which is speculated to be due to the actual age of her DNA. Because the DNA in cloned cells belongs to an older individual,  the cloned individual’s life expectancy may be affected. Indeed, analysis of Dolly’s DNA revealed shorter...
8.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

RIN4 fragments released by AvrRpt2 promote NDR1-dependent activation of RPS2.

The Plant cell·2026
Same author

Cytokinin-mediated trichome initiation in Nicotiana benthamiana following Agrobacterium tumefaciens infiltration.

Plant communications·2026
Same author

Starch and tuber traits of diploid potato lines B26 and B100 and their hybrids.

BMC plant biology·2026
Same author

A helper NLR channels organellar calcium to trigger plant immunity.

Science (New York, N.Y.)·2026
Same author

Transcriptional profiling of circulating extracellular vesicles from prebiopsy prostate cancer patients.

Molecular oncology·2026
Same author

An activated wheat CC<sub>G10</sub>-NLR immune receptor forms an octameric resistosome.

Cell·2026

Related Experiment Video

Updated: Apr 28, 2026

Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit
06:28

Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit

Published on: September 2, 2025

1.0K

A golden gate modular cloning toolbox for plants.

Carola Engler1, Mark Youles, Ramona Gruetzner

  • 1Icon Genetics GmbH, Weinbergweg 22, Biozentrum Halle, 06120 Halle, Germany.

ACS Synthetic Biology
|June 17, 2014
PubMed
Summary
This summary is machine-generated.

Plant synthetic biology needs efficient gene assembly. This study introduces a Golden Gate cloning resource with standardized parts and vectors for creating multigene constructs in plants, simplifying complex genetic engineering.

Keywords:
Agrobacterium tumefaciensgene assemblygenetic engineeringmolecular cloningplant biotechnologyvector construction

More Related Videos

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
08:31

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

13.9K
Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit
08:25

Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit

Published on: October 31, 2019

16.1K

Related Experiment Videos

Last Updated: Apr 28, 2026

Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit
06:28

Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit

Published on: September 2, 2025

1.0K
Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
08:31

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

13.9K
Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit
08:25

Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit

Published on: October 31, 2019

16.1K

Area of Science:

  • Plant Synthetic Biology
  • Molecular Biology
  • Genetics

Background:

  • Efficient assembly of multigene constructs is crucial for advancing plant synthetic biology.
  • Traditional cloning methods can be cumbersome for complex genetic engineering tasks.
  • Golden Gate cloning offers a modular and precise approach for DNA assembly.

Purpose of the Study:

  • To develop and present a versatile resource for plant synthetic biology.
  • To enable the facile construction of multigene constructs using Golden Gate cloning.
  • To provide standardized parts for plant transformation vectors.

Main Methods:

  • Development of a set of Golden Gate cloning vectors.
  • Creation of 96 standardized DNA parts (promoters, terminators, reporters, etc.).
  • Testing and comparative performance analysis of parts in the model plant *Nicotiana benthamiana*.

Main Results:

  • A comprehensive resource of vectors and standardized parts for Golden Gate assembly is established.
  • The utility of the resource for constructing multigene constructs for plant transformation is demonstrated.
  • Comparative performance data for various parts in *Nicotiana benthamiana* is provided.

Conclusions:

  • The presented resource significantly facilitates the assembly of multigene constructs in plant synthetic biology.
  • Standardized parts and Golden Gate cloning streamline the engineering of complex plant genetic systems.
  • This toolkit empowers researchers to accelerate advancements in plant biotechnology.