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Pressure-voltage trap for DNA near a solid-state nanopore.

David P Hoogerheide1, Bo Lu, Jene A Golovchenko

  • 1Department of Physics and ‡School of Engineering and Applied Sciences, Harvard University , Cambridge, Massachusetts 02138, United States.

ACS Nano
|June 17, 2014
PubMed
Summary

We developed a tunable trap for single DNA molecules near a solid-state nanopore. This trap allows molecules to repeatedly enter and exit the pore, revealing detailed fluctuation dynamics.

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Area of Science:

  • Biophysics
  • Nanotechnology
  • Physical Chemistry

Background:

  • Single molecule manipulation is crucial for understanding DNA dynamics.
  • Solid-state nanopores offer precise control over molecular confinement.
  • Balancing forces is key to stable molecular trapping.

Purpose of the Study:

  • To create a tunable trap for single DNA molecules near a solid-state nanopore.
  • To investigate the dynamics of DNA molecules under balanced electric and flow forces.
  • To model the fluctuation phenomena of trapped DNA molecules.

Main Methods:

  • Utilizing a solid-state nanopore in an electrolyte solution.
  • Applying balanced electric and pressure-induced viscous flow forces.
  • Statistical analysis of individually trapped DNA molecules.
  • Employing a one-dimensional first passage model.

Main Results:

  • Achieved stable trapping of single DNA molecules.
  • Observed multiple entries and exits of trapped DNA molecules.
  • Detailed characterization of fluctuation phenomena.
  • Successful modeling of trapping dynamics using a first passage approach.

Conclusions:

  • The developed trap enables precise control and observation of single DNA molecule dynamics.
  • The balanced force conditions facilitate detailed studies of molecular fluctuations.
  • First passage modeling accurately describes the observed trapping and escape behaviors.