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Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

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Related Experiment Video

Updated: Apr 28, 2026

CcCIPK14 Gene Function Analysis to Illuminate the Efficient Root Transgenic System

CcCIPK14 Gene Function Analysis to Illuminate the Efficient Root Transgenic System

Published on: September 23, 2021

An efficient Agrobacterium-mediated transformation system for poplar.

Ali Movahedi¹, Jiaxin Zhang², Rasoul Amirian³

¹The Cooperative Innovation Center of Southern Modern Forestry, Nanjing Forestry University, Nanjing 210037, China. ali664699@gmail.com.

International Journal of Molecular Sciences

|June 17, 2014

Summary

Optimizing Agrobacterium tumefaciens transformation in poplar (Populus) significantly improved genetic modification efficiency. Key factors include specific suspension conditions, infection duration, acetosyringone concentration, and cocultivation parameters for faster transgenic studies.

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Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain

Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain

Published on: March 17, 2023

Area of Science:

Plant Biotechnology
Molecular Biology
Forestry Science

Background:

Poplar (Populus) is a vital model for woody plant regeneration and genetic transformation.
Accelerating transgenic poplar studies requires optimizing Agrobacterium tumefaciens-mediated transformation methods.

Purpose of the Study:

To enhance the efficiency of Agrobacterium tumefaciens-mediated genetic transformation in poplar.
To identify key factors influencing transformation success in Populus hybrid clone "Nanlin895".

Main Methods:

Optimization of Agrobacterium tumefaciens suspension parameters (OD600, pH, pre-treatment).
Screening of infection duration, acetosyringone concentration, and cocultivation conditions (temperature, duration, medium composition).
Evaluation of explant preculture and confirmation of gene integration and expression via Southern and Northern blot analyses.

Main Results:

Transformation efficiency was significantly improved by optimizing multiple factors.
Specific conditions identified include OD600 of 0.7, 120 min infection, pH 5.0, 200 µM acetosyringone, 28 °C for 72 h cocultivation, and 30 g/L sucrose.
Preculture of wounded leaf explants for two days enhanced the regeneration rate.

Conclusions:

The study presents a significantly optimized protocol for Agrobacterium-mediated transformation of poplar.
This optimized method accelerates the generation of transgenic poplar lines for research.
Confirmed gene integration and expression in regenerated transgenic poplars.