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Updated: Mar 2, 2026
Author Spotlight: Ex Vivo Protocol for Culturing Quiescent Muscle Stem Cells with Niche Components
Published on: June 2, 2023
Embryonic stem cells improve skeletal muscle recovery after extreme atrophy in mice
Guilherme Giannini Artioli1, João Guilherme De Oliveira Silvestre, João Paulo Limongi França Guilherme
1Laboratory of Cellular and Molecular Biology of Striated Muscle, Department of Anatomy, Institute of Biomedical Sciences, Avenida Prof. Lineu Prestes 2415, São Paulo CEP 05508-000, Brazil; Laboratory of Applied Nutrition and Metabolism, School of Physical Education and Sport, Department of Biodynamics, University of São Paulo, São Paulo, SP, Brazil.
Introduction:
We injected embryonic stem cells into mouse tibialis anterior muscles subjected to botulinum toxin injections as a model for reversible neurogenic atrophy.
Methods:
Muscles were exposed to botulinum toxin for 4 weeks and allowed to recover for up to 6 weeks. At the onset of recovery, a single muscle injection of embryonic stem cells was administered. The myofiber cross-sectional area, single twitch force, peak tetanic force, time-to-peak force, and half-relaxation time were determined.
Results:
Although the stem cell injection did not affect the myofiber cross-sectional area gain in recovering muscles, most functional parameters improved significantly compared with those of recovering muscles that did not receive the stem cell injection.
Conclusions:
Muscle function recovery was accelerated by embryonic stem cell delivery in this durable neurogenic atrophy model. We conclude that stem cells should be considered a potential therapeutic tool for recovery after extreme skeletal muscle atrophy.
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