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Updated: Apr 28, 2026

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
Published on: August 21, 2016
[Searching for new antibiotics--inhibitors of bacterial chromosome replication]
Damian Trojanowski1, Patrycja Skut1, Joanna Hołówka2
1Wydział Biotechnologii, Uniwersytet Wrocławski.
Abstract:
The excessive and often unreasonable use of antibacterial drugs leads to rise of antibioticresistant strains. To overcome this problem, new antibiotics are searched and the new drug targets are investigated. The proteins involved in replication of bacterial chromosomes seem to be promising candidates for drug targets since they are involved in crucial life pathways and are structurally and/or functionally different from the eukaryotic homologues. Within last few years, a large number of newly developed methods allowed to search among thousands of molecules for the ones that specifically inhibit DNA synthesis in the prokaryotic cell. In this review, we present some of these methods.
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