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Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling
Published on: March 20, 2018
Diarylheptanoids from green alder bark and their potential for DNA protection
Miroslav Novaković1, Miroslava Stanković, Ivan Vučković
1Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Studentski trg 16, RS-11000 Belgrade (phone: +381-11-2630474; fax: +381-11-2636061). mironov76@yahoo.com.
Abstract:
Nine diarylheptanoids, 1-9, catechin (11), and a phenolic glucoside, 10, were isolated from the bark of green alder (Alnus viridis). Four of the isolated compounds, i.e., 2, 5, 8, 10, are new. The structures of 1-11 were determined on the basis of spectroscopic data. All isolated compounds were evaluated for their in vitro protective effects on chromosome aberrations in peripheral human lymphocytes using cytokinesis-block micronucleus (CBMN) assay. Almost all of them exerted a pronounced effect of decreasing DNA damage of human lymphocytes, acting stronger than the known synthetic protector amifostine.
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