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Polyadenylation of Friend murine leukemia virus env-mRNA is affected by its splicing

Akihito Machinaga1, Sayaka Takase-Yoden

  • 1Department of Bioinformatics, Faculty of Engineering, Soka University, 1-236, Tangi-machi, Hachioji-shi, Tokyo, 192-8577, Japan.

Microbiology and Immunology
|June 18, 2014
PubMed

Insights

Splicing of Friend murine leukemia virus env-mRNA is crucial for efficient polyadenylation, enhancing Env protein expression. This study reveals splicing

Area of Science:

  • Molecular Biology
  • Virology
  • Gene Expression

Background:

  • Splicing of Friend murine leukemia virus (FMLV) env-mRNA enhances its stability and translation.
  • The role of splicing in the polyadenylation of env-mRNA was not previously understood.

Purpose of the Study:

  • To investigate the effect of FMLV env-mRNA splicing on poly(A) tail length.
  • To determine if splicing influences the efficiency of env-mRNA polyadenylation.

Main Methods:

  • Utilized env expression vectors to generate both unspliced and spliced env-mRNA.
  • Analyzed poly(A) tail length of FMLV env-mRNA products.

Main Results:

  • Incomplete polyadenylation was observed in a subset of unspliced env-mRNA.
  • This incomplete polyadenylation occurred in an env gene-dependent manner.
  • Splicing of FMLV env-mRNA significantly improved the efficiency of complete polyadenylation.

Conclusions:

  • FMLV env-mRNA splicing is essential for efficient and complete polyadenylation.
  • Splicing-mediated promotion of polyadenylation may contribute to the observed up-regulation of Env protein expression.
Keywords:
env mRNAmurine leukemia viruspoly(A) tailsplicing

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