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Related Experiment Video

Updated: Apr 28, 2026

In vivo Interrogation of Central Nervous System Translatome by Polyribosome Fractionation

In vivo Interrogation of Central Nervous System Translatome by Polyribosome Fractionation

Published on: April 30, 2014

The neuronal activity-driven transcriptome.

Eva Benito¹, Angel Barco

¹Instituto de Neurociencias (Universidad Miguel Hernández-Consejo Superior de Investigaciones Científicas), Av. Santiago Ramón y Cajal s/n. Sant Joan d'Alacant, 03550, Alicante, Spain.

Molecular Neurobiology

|June 18, 2014

Summary

This summary is machine-generated.

Neuronal plasticity relies on activity-driven transcription. This review explores "omics" approaches to understand gene regulation by transcription factors (e.g., CREB, SRF) and epigenetic mechanisms in neurons.

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Area of Science:

Neuroscience
Molecular Biology
Genetics

Background:

Activity-driven transcription is crucial for long-lasting neuronal plasticity.
Understanding the systems biology of this process and the roles of specific transcription factors remains limited.

Purpose of the Study:

To review neuronal activity-driven transcription from an "omics" perspective.
To discuss high-throughput methods for characterizing gene programs regulated by transcription factors like CREB, SRF, MeCP2, Fos, and Npas4.
To explore the interplay between epigenetic and transcriptional mechanisms in neuronal plasticity.

Main Methods:

Review of high-throughput "omics" approaches.
Analysis of genome-wide techniques for studying neuronal transcriptome and epigenome.
Focus on activity-regulated transcription factors and their downstream gene programs.

Main Results:

Identified key activity-regulated transcription factors (CREB, SRF, MeCP2, Fos, Npas4) and their roles.
Highlighted the interplay between epigenetic modifications and transcriptional regulation.
Emphasized the contribution of "omics" to understanding gene programs in neuronal plasticity.

Conclusions:

Significant advances have been made in understanding neuronal activity-driven transcription.
Genome-wide techniques offer promising avenues for future research.
Further investigation is needed to fully elucidate the systems biology of neuronal plasticity.