Related Experiment Video
Updated: Apr 28, 2026

In Situ Hybridization Techniques for Paraffin-Embedded Adult Coral Samples
Published on: August 31, 2018
Gene co-expression modules underlying polymorphic and monomorphic zooids in the colonial hydrozoan, Hydractinia
David C Plachetzki1, M Sabrina Pankey2, Brian R Johnson2
1*Department of Molecular, Cellular and Biomedical Sciences, The University of New Hampshire, Durham, NH 03801, USA; Department of Ecology Evolution and Marine Biology, The University of California at Santa Barbara, Santa Barbara, CA 93106, USA; Department of Entomology, The University of California at Davis, Davis, CA 95616, USA; Department of Evolution and Ecology, Center for Population Biology, The University of California at Davis, Davis, CA 95616, USA david.plachetzki@unh.edu.
Abstract:
Advances in sequencing technology have forced a quantitative revolution in Evolutionary Biology. One important feature of this renaissance is that comprehensive genomic resources can be obtained quickly for almost any taxon, thus speeding the development of new model organisms. Here, we analyze 20 RNA-seq libraries from morphologically, sexually, and genetically distinct polyp types from the gonochoristic colonial hydrozoan, Hydractinia symbiolongicarpus (Cnidaria). Analyses of these data using weighted gene co-expression networks highlight deeply conserved genetic elements of animal spermatogenesis and demonstrate the utility of these methods in identifying modules of genes that correlate with different zooid types across various statistical contrasts. RNA-seq data and analytical scripts described here are deposited in publicly available databases.
Related Concept Videos
Multipotency and Niche of Bulge Stem Cell
Red Algae
Constitutive and Regulated Gene Expression
Cell Specific Gene Expression
Cell Specific Gene Expression
Zygotic Development And Stem Cell Formation

