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Updated: Apr 28, 2026

Expression and Purification of Virus-like Particles for Vaccination

Expression and Purification of Virus-like Particles for Vaccination

Published on: June 2, 2016

Alphavirus-based vaccines.

Kenneth Lundstrom¹

¹PanTherapeutics, Rue des Remparts 4, CH1095 Lutry, Switzerland. lundstromkenneth@gmail.com.

|June 18, 2014

Summary

This summary is machine-generated.

Alphavirus vectors offer promising vaccine development potential, showing strong gene expression and robust protection against viral challenges and tumors in animal models. Clinical trials are now underway for human applications.

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Area of Science:

Virology
Immunology
Biotechnology

Background:

Alphavirus vectors are recognized for high transient gene expression in vitro and in vivo.
Common vectors derive from Semliki Forest virus, Sindbis virus, and Venezuelan equine encephalitis virus.
Applications include replication-deficient and replication-proficient particles, in vitro transcribed RNA, and DNA vectors.

Purpose of the Study:

To review the development and applications of alphavirus vectors for vaccine strategies.
To highlight the immunogenicity and protective efficacy of alphavirus vector-based vaccines.
To discuss the potential for alphavirus vectors in both infectious disease and cancer immunotherapy.

Main Methods:

Utilizing alphavirus vectors for the expression of heterologous genes, including viral structural proteins and tumor antigens.
Administering these vectors in various formats (recombinant particles, RNA, DNA) for immunization studies.
Evaluating immune responses, such as neutralizing antibody titers, and protective efficacy in preclinical animal models (rodents, primates).

Main Results:

Alphavirus vectors successfully expressed immunogenic proteins, eliciting strong neutralizing antibody responses.
Immunization conferred robust protection against lethal viral challenges in rodents and primates.
Vaccination with alphavirus vectors expressing tumor antigens provided prophylactic protection against tumor cells.

Conclusions:

Alphavirus vectors are effective tools for vaccine development, demonstrating significant immunogenicity and protective capabilities.
Their versatility supports applications against infectious diseases and cancer.
Advancements have progressed to the stage of human clinical trials.