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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

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Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
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Atomic Fluorescence Spectroscopy01:29

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Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which...
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Overview of Microscopy Techniques01:22

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The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
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Related Experiment Video

Updated: Apr 28, 2026

High-Speed Atomic Force Microscopy Imaging of DNA Three-Point-Star Motif Self Assembly Using Photothermal Off-Resonance Tapping
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High-speed atomic force microscopy: imaging and force spectroscopy.

Frédéric Eghiaian1, Felix Rico1, Adai Colom1

  • 1U1006 INSERM, Aix-Marseille Université, Parc Scientifique et Technologique de Luminy, 163 avenue de Luminy, 13009 Marseille, France.

FEBS Letters
|June 18, 2014
PubMed
Summary
This summary is machine-generated.

High-speed atomic force microscopy (HS-AFM) offers sub-second, nanometer-resolution imaging of biological samples. This technique provides new insights into molecular dynamics and protein unfolding, complementing existing microscopy methods.

Keywords:
Actin cortexHigh-speed atomic force microscopyHigh-speed force spectroscopyMembrane proteinMembrane structureTitin

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Area of Science:

  • Biophysics
  • Molecular Biology
  • Microscopy

Background:

  • Atomic force microscopy (AFM) excels at imaging biological samples in physiological conditions with high resolution.
  • AFM is valuable for studying protein unfolding and cellular force spectroscopy.
  • Despite its capabilities, AFM is less popular than electron or fluorescence microscopy.

Purpose of the Study:

  • To review recent advancements in high-speed atomic force microscopy (HS-AFM) for dynamic bio-molecular imaging.
  • To highlight the potential of HS-AFM in revealing single-molecule dynamics.
  • To discuss the emergence of high-speed force spectroscopy (HS-FS) for protein unfolding studies.

Main Methods:

  • High-speed atomic force microscopy (HS-AFM) for dynamic imaging.
  • High-speed force spectroscopy (HS-FS) for mechanical analysis.
  • Review of recent scientific reports utilizing HS-AFM and HS-FS.

Main Results:

  • HS-AFM provides unprecedented insights into the dynamics of membrane proteins and molecular machines.
  • HS-AFM achieves nanometer-resolution and sub-second frame rates for imaging.
  • Recent reports showcase dynamic bio-molecular imaging and single protein unfolding studies using HS-AFM and HS-FS.

Conclusions:

  • HS-AFM opens new research avenues for depicting dynamic events at the single bio-molecule level.
  • HS-AFM is a valuable complementary technique to other structural and cellular biology methods.
  • Increased acceptance of HS-AFM is anticipated among researchers in various scientific fields.