Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electrophoresis: Overview01:20

Electrophoresis: Overview

4.1K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
4.1K
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

1.9K
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
1.9K
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

1.8K
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
1.8K
Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

6.0K
Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
6.0K
SDS-PAGE01:27

SDS-PAGE

23.0K
Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
23.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spatially Profiling Trace Cytokine Signatures From Microscopically Derived Skin Samples to Probe Skin Disease Inflammation.

Small methods·2026
Same author

Mechanism of peak fronting in ion exclusion chromatography.

Journal of chromatography. A·2026
Same author

Multifactor authentication in extracellular vesicle analysis: methods and approaches to address the heterogeneity problem.

Nature methods·2026
Same author

Glycaemic variability underlies myocyte dysfunction and myocardial injury risk in diabetes.

Nature communications·2026
Same author

Nanobiosensors and Artificial Intelligence Strategies for Glycan Profiling in Cancer Progression: A Critical Review.

ACS sensors·2026
Same author

Early-stage multi-cancer detection through a plasma extracellular vesicle protein signature.

Cell reports. Medicine·2026
Same journal

Advances in application of microplasmas for non-metallic species analysis by optical spectrometry.

The Analyst·2026
Same journal

Sulfur vacancy-mediated self-photocatalysis-boosted electrochemiluminescence sensing <i>via</i> molecular oxygen activation for sensitive detection of isocarbophos.

The Analyst·2026
Same journal

Analytical challenges in mapping the subcellular metabolome and lipidome.

The Analyst·2026
Same journal

Threshold-guided multiplex PCR-LFA: a step toward UTI pathogen detection.

The Analyst·2026
Same journal

Aptamer-based CRISPR-Cas12a fluorescent biosensors for serum biomarker detection.

The Analyst·2026
Same journal

A two-step centrifugal microfluidic platform for semi-automated IGRA detection of tuberculosis based on chemiluminescence.

The Analyst·2026
See all related articles

Related Experiment Video

Updated: Apr 28, 2026

Analysis of AtHIRD11 Intrinsic Disorder and Binding Towards Metal Ions by Capillary Gel Electrophoresis and Affinity Capillary Electrophoresis
07:54

Analysis of AtHIRD11 Intrinsic Disorder and Binding Towards Metal Ions by Capillary Gel Electrophoresis and Affinity Capillary Electrophoresis

Published on: August 22, 2018

5.5K

Zero net-flow in capillary electrophoresis using acrylamide based hydrogel.

Alain Wuethrich1, Paul R Haddad, Joselito P Quirino

  • 1Australian Centre for Research on Separation Science (ACROSS), School of Physical Sciences-Chemistry, University of Tasmania, Private Bag 75, Hobart, TAS 7001, Australia. jquirino@utas.edu.au.

The Analyst
|June 19, 2014
PubMed
Summary
This summary is machine-generated.

Acrylamide hydrogels enabled zero net-flow in capillary electrophoresis. This technique allows for the sensitive detection and injection of anionic compounds using field-enhanced sample injection in counter-electroosmotic flow.

More Related Videos

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time
11:05

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time

Published on: October 26, 2016

8.2K
CN-GELFrEE - Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis
11:38

CN-GELFrEE - Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis

Published on: February 29, 2016

11.3K

Related Experiment Videos

Last Updated: Apr 28, 2026

Analysis of AtHIRD11 Intrinsic Disorder and Binding Towards Metal Ions by Capillary Gel Electrophoresis and Affinity Capillary Electrophoresis
07:54

Analysis of AtHIRD11 Intrinsic Disorder and Binding Towards Metal Ions by Capillary Gel Electrophoresis and Affinity Capillary Electrophoresis

Published on: August 22, 2018

5.5K
Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time
11:05

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time

Published on: October 26, 2016

8.2K
CN-GELFrEE - Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis
11:38

CN-GELFrEE - Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis

Published on: February 29, 2016

11.3K

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Materials Science

Background:

  • Capillary electrophoresis (CE) is a powerful separation technique.
  • Controlling electroosmotic flow (EOF) is crucial for optimizing CE separations.
  • Acrylamide-based hydrogels offer tunable properties for flow manipulation.

Purpose of the Study:

  • To investigate the use of acrylamide-based hydrogels for achieving zero net-flow in CE.
  • To develop a method for detecting anionic compounds using counter-electroosmotic flow (CEOF).
  • To demonstrate field-enhanced sample injection (FESI) for anionic small molecule drugs.

Main Methods:

  • Utilized acrylamide-based hydrogel in a fused-silica capillary.
  • Operated capillary electrophoresis with electroosmotic flow.
  • Employed counter-electroosmotic flow (CEOF) with the anode at the detector end.
  • Implemented field-enhanced sample injection (FESI).

Main Results:

  • Achieved zero net-flow conditions using the acrylamide-based hydrogel.
  • Successfully detected anionic compounds under CEOF conditions.
  • Demonstrated effective FESI for anionic small molecule drug analysis.

Conclusions:

  • Acrylamide-based hydrogels can effectively establish zero net-flow in CE.
  • The developed CEOF-CE method with FESI is suitable for anionic compound detection.
  • This approach offers a novel strategy for analyzing anionic small molecules.