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Reprocessing unsatisfactory ThinPrep papanicolaou tests using a modified SurePath preparation technique.

Melissa L Randolph, Howard H Wu, William N Crabtree

    Cancer Cytopathology
    |June 19, 2014
    PubMed
    Summary
    This summary is machine-generated.

    A modified SurePath reprocessing technique effectively improved unsatisfactory gynecologic specimens, converting 56% to satisfactory. This method successfully identified 116 abnormalities, including high-grade squamous intraepithelial lesions, that would have otherwise been missed.

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    Area of Science:

    • Cytopathology
    • Gynecologic Pathology
    • Specimen Processing

    Background:

    • Increasing rates of unsatisfactory gynecologic specimens due to lubricants, blood, and debris.
    • Challenges in cell transference caused by clogged filters in liquid-based cytology.
    • Need for improved methods to ensure specimen adequacy for accurate diagnosis.

    Purpose of the Study:

    • To evaluate a modified SurePath reprocessing technique for improving unsatisfactory ThinPrep Papanicolaou tests.
    • To assess the technique's efficacy in overcoming common specimen contaminants.
    • To determine the impact of reprocessing on the detection of epithelial cell abnormalities.

    Main Methods:

    • Reprocessing of unsatisfactory ThinPrep specimens using a modified SurePath technique.
    • Inclusion of methanol and Tris-buffered washes to remove contaminants.
    • Manual review of both original and reprocessed specimens by cytotechnologists and pathologists.

    Main Results:

    • 1093 (56%) of 1937 reprocessed specimens were converted to a satisfactory state.
    • Transformation zone component was present in 624 (57%) of satisfactory specimens.
    • 116 epithelial cell abnormalities (10.6%) were identified, including 11 high-grade squamous intraepithelial lesions.

    Conclusions:

    • The modified SurePath technique effectively addresses challenges from blood, protein, and lubricants in liquid-based preparations.
    • Reprocessing converted a significant number of unsatisfactory specimens to satisfactory, enabling detection of critical abnormalities.
    • This technique enhances diagnostic yield by salvaging specimens previously deemed inadequate.