Radio-isotopic determination of subnanomolar amounts of peroxide

A new radio-isotopic method for determination of peroxide in subnanomolar concentrations has been described. The method is based on the reactivity of peroxide with alpha-Ketoglutaric acid containing [I-14C]-alpha-Ketoglutaric acid, and measurement of the resulting 14CO2 by radioactivity. The recovery of standard peroxide, alone or mixed with some biologically derived samples, following this technique was found to be 97 +/- 2.7%. The method may therefore be useful for determination of peroxides in active biological samples.