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[Down-regulation effect of TGF-beta1 on expression of VEGF in bladder cancer cell line]
Objective:
To explore the down-regulation effect of transforming growth factor-beta1 (TGF-beta1) on the expression of vascular endothelial growth factor (VEGF) in bladder cancer cell line EJ cell strain.
Methods:
The siRNA expression vectors of TGF-beta1, gene were constructed, the highest inhibition target sequence was screened and selected by real time-PCR and ELISA, and then the vectors were transfected into EJ cells, the expression level of VEGF mRNA was detected by Real time-PCR.
Results:
TGF-beta1 targeting expression vectors were successfully constructed, Real time-PCR and ELISA screened the highest inhibition target sequence (the lowest expression level of TGF-beta1 mRNA was 0.92 +/- 0.19; ELISA result was 50.08 +/- 5.85). The relative expression level of VEGF mRNA in TGF-beta1 siRNA group was the lowest, indicating that the expression of VEGF was decreased by silencing TGF-beta1, gene.
Conclusion:
Inhibition of TGF-beta1 gene expression could down regulate the expression of VEGF in bladder neoplasm.
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