Related Experiment Video
Updated: Apr 28, 2026

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions
Published on: September 30, 2016
Expression patterns of chitinase and chitosanase produced from Bacillus cereus in suppression of phytopathogen
Dong-Jun Seo1, Ji-Hae Lee1, Yong-Su Song1
1Division of Applied Bioscience and Biotechnology, Institute of Environmentally-Friendly Agriculture (IEFA), College of Agricultural and Life Science, Chonnam National University, Gwangju 500-757, Republic of Korea.
Abstract:
Bacillus cereus MP-310 was incubated on various culture media substrates as LB, colloidal chitin, chitosan powder, and chitosan beads to investigate the concurrent expression patterns of chitinase and chitosanase isozymes by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Chitinase activity increased rapidly with a maximum level after 6 days of incubation in CM-chitin medium. Major bands of chitinase isozymes were strongly expressed on SDS-PAGE in LB medium (four bands) and in colloidal chitin medium (five bands) after 6 days after incubation, and in chitosan powder medium (one band) and in chitosan beads medium (five bands) after 12 days after incubation. A major band of chitosanase isozymes was strongly expressed on SDS-PAGE in chitosan powder medium (one band) and in chitosan beads medium (one band) after 12 days of incubation.
Related Concept Videos
Production of Biopesticides
Biological Methods for Microbial Control
Regulation of Bacterial Virulence
Gene Regulation in Microbial Communities: Quorum Sensing

