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Human monocytes can produce tissue-type plasminogen activator
P H Hart1, G F Vitti, D R Burgess
1Department of Medicine, University of Melbourne, Royal Melbourne Hospital, Parkville, Australia.
The Journal of Experimental Medicine
|April 1, 1989
Summary
Human monocytes can produce tissue-type plasminogen activator (t-PA) when stimulated in vitro. This finding was confirmed by detecting t-PA messenger RNA (mRNA) in these cells.
Area of Science:
- Immunology
- Molecular Biology
- Biochemistry
Background:
- Monocytes and macrophages are known to produce urokinase-type plasminogen activator.
- The production of tissue-type plasminogen activator (t-PA) by human monocytes has not been previously established.
Purpose of the Study:
- To investigate the potential of human monocytes to produce tissue-type plasminogen activator (t-PA).
- To characterize the t-PA produced by stimulated human monocytes in vitro.
Main Methods:
- In vitro stimulation of human monocytes.
- Biochemical and immunological characterization of produced plasminogen activator.
- Detection of messenger RNA (mRNA) for t-PA.
Main Results:
- Human monocytes, upon appropriate in vitro stimulation, were demonstrated to produce a 70 kD form of tissue-type plasminogen activator (t-PA).
- The presence of t-PA mRNA in stimulated monocytes confirmed the biochemical and immunological findings.
- This study provides the first evidence of t-PA production by human monocytes.
Conclusions:
- Human monocytes are a source of tissue-type plasminogen activator (t-PA).
- The production of t-PA by monocytes can be induced under specific in vitro conditions.
- These findings contribute to understanding the role of monocytes in fibrinolysis.