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Updated: Apr 28, 2026

High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension
Published on: December 28, 2015
Expression, purification, and immobilization of recombinant tamavidin 2 fusion proteins
Yoshimitsu Takakura1, Naomi Oka, Masako Tsunashima
1Plant Innovation Center, Japan Tobacco, Inc., 700 Higashibara, Iwata, Shizuoka, 438-0802, Japan, yoshimitsu.takakura@jt.com.
Abstract:
Tamavidin 2 is a fungal avidin-like protein that binds biotin with high affinity. Unlike avidin or streptavidin, tamavidin 2 in soluble form is produced at high levels in Escherichia coli. In this chapter, we describe a method for immobilization and purification of recombinant proteins with the use of tamavidin 2 as an affinity tag. The protein fused to tamavidin 2 is tightly immobilized and simultaneously purified on biotinylated magnetic microbeads without loss of activity.
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